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High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers.

ABSTRACT: BACKGROUND:Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. RESULTS:We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes. CONCLUSIONS:The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.

SUBMITTER: Gustafsson C

PROVIDER: S-EPMC6339279 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers.

Gustafsson Charlotte C De Paepe Ayla A Schmidl Christian C Månsson Robert R

BMC genomics 20190118 1

<h4>Background</h4>Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level.<h4>Results</h4>We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes.<h4>Conclusions</h4>The resulting workflow is easily established, extremely rapid, and compatible with re ...[more]

PMID: 30658577

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