Ontology highlight
ABSTRACT: Background
The malaria causing parasite Plasmodium subverts host immune responses by several strategies including the modulation of dendritic cells (DCs).Methods
In this study, we show that Plasmodium falciparum skewed CD16+ DC cytokine responses towards interleukin (IL)-10 production in vitro, distinct to the cytokine profile induced by Toll-like receptor ligation. To determine CD16+ DC responsiveness in vivo, we assessed their function after induced P falciparum infection in malaria-naive volunteers.Results
CD16+ DCs underwent distinctive activation, with increased expression of maturation markers human leukocyte antigen (HLA)-DR and CD86, enhanced tumor necrosis factor (TNF) production, and coproduction of TNF/IL-10. In vitro restimulation with P falciparum further increased IL-10 production. In contrast, during naturally acquired malaria episode, CD16+ DCs showed diminished maturation, suggesting increased parasite burden and previous exposure influence DC subset function.Conclusions
These findings identify CD16+ DCs as the only DC subset activated during primary blood-stage human Plasmodium infection. As dual cytokine producers, CD16+ DCs contribute to inflammatory as well as regulatory innate immune processes.
SUBMITTER: Loughland JR
PROVIDER: S-EPMC6339523 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Loughland Jessica R JR Woodberry Tonia T Boyle Michelle J MJ Tipping Peta E PE Piera Kim A KA Amante Fiona H FH Kenangalem Enny E Price Ric N RN Engwerda Christian R CR Anstey Nicholas M NM McCarthy James S JS Minigo Gabriela G
The Journal of infectious diseases 20190101 4
<h4>Background</h4>The malaria causing parasite Plasmodium subverts host immune responses by several strategies including the modulation of dendritic cells (DCs).<h4>Methods</h4>In this study, we show that Plasmodium falciparum skewed CD16+ DC cytokine responses towards interleukin (IL)-10 production in vitro, distinct to the cytokine profile induced by Toll-like receptor ligation. To determine CD16+ DC responsiveness in vivo, we assessed their function after induced P falciparum infection in ma ...[more]