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Activation of silent biosynthetic gene clusters using transcription factor decoys.

ABSTRACT: Here we report a transcription factor decoy strategy for targeted activation of eight large silent polyketide synthase and non-ribosomal peptide synthetase gene clusters, ranging from 50 to 134 kilobases (kb) in multiple streptomycetes, and characterization of a novel oxazole family compound produced by a 98-kb biosynthetic gene cluster. Owing to its simplicity and ease of use, this strategy can be scaled up readily for discovery of natural products in streptomycetes.

SUBMITTER: Wang B

PROVIDER: S-EPMC6339570 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Activation of silent biosynthetic gene clusters using transcription factor decoys.

Wang Bin B Guo Fang F Dong Shi-Hui SH Zhao Huimin H

Nature chemical biology 20181231 2

Here we report a transcription factor decoy strategy for targeted activation of eight large silent polyketide synthase and non-ribosomal peptide synthetase gene clusters, ranging from 50 to 134 kilobases (kb) in multiple streptomycetes, and characterization of a novel oxazole family compound produced by a 98-kb biosynthetic gene cluster. Owing to its simplicity and ease of use, this strategy can be scaled up readily for discovery of natural products in streptomycetes. ...[more]

PMID: 30598544

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