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Structural basis of DNA lesion recognition for eukaryotic transcription-coupled nucleotide excision repair.

ABSTRACT: Eukaryotic transcription-coupled nucleotide excision repair (TC-NER) is a pathway that removes DNA lesions capable of blocking RNA polymerase II (Pol II) transcription from the template strand. This process is initiated by lesion-arrested Pol II and the recruitment of Cockayne Syndrome B protein (CSB). In this review, we will focus on the lesion recognition steps of eukaryotic TC-NER and summarize the recent research progress toward understanding the structural basis of Pol II-mediated lesion recognition and Pol II-CSB interactions. We will discuss the roles of CSB in both TC-NER initiation and transcription elongation. Finally, we propose an updated model of tripartite lesion recognition and verification for TC-NER in which CSB ensures Pol II-mediated recognition of DNA lesions for TC-NER.

SUBMITTER: Wang W

PROVIDER: S-EPMC6340766 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Structural basis of DNA lesion recognition for eukaryotic transcription-coupled nucleotide excision repair.

Wang Wei W Xu Jun J Chong Jenny J Wang Dong D

DNA repair 20180823

Eukaryotic transcription-coupled nucleotide excision repair (TC-NER) is a pathway that removes DNA lesions capable of blocking RNA polymerase II (Pol II) transcription from the template strand. This process is initiated by lesion-arrested Pol II and the recruitment of Cockayne Syndrome B protein (CSB). In this review, we will focus on the lesion recognition steps of eukaryotic TC-NER and summarize the recent research progress toward understanding the structural basis of Pol II-mediated lesion re ...[more]

PMID: 30174298

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