Dataset Information

Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion.

ABSTRACT:

Background

Plasmodium vivax malaria elimination can only be achieved by the deployment of 8-aminoquinolines (primaquine and tafenoquine) in combination with ACT to kill both blood and liver-stage parasites. However, primaquine and the other 8-aminoquinolines cause dose-dependent haemolysis in subjects with G6PD deficiency, an X-linked disorder of red blood cells that is very common in populations living in tropical and subtropical areas. In order to inform safer use of 8-aminoquinolines in the Greater Mekong Subregion, a multi-centre study was carried out to assess the prevalence of G6PD deficiency and to identify the main G6PD variants in samples collected in Cambodia, Lao PDR, Myanmar, Thailand and Vietnam.

Methods

Blood samples were collected in the five countries during National Malaria Surveys or during Population Surveys. During Population Surveys samples were characterized for G6PD phenotype using the Fluorescent Spot Test. Samples were then genotyped for a panel of G6PD mutations.

Results

G6PD deficiency was found to be common in the region with an overall mean prevalence of deficient or mutated hemizygous males of 14.0%, ranging from a mean 7.3% in Thailand, 8.1% in Lao PDR, 8.9% in Vietnam, 15.8% in Myanmar and 18.8% in Cambodia. Mahidol and Viangchan mutations were the most common and widespread variants found among the nine investigated.

Conclusions

Owing to the high prevalence of G6PD deficiency in the Greater Mekong Subregion, strategies for vivax malaria elimination should include point-of-care G6PD testing (both qualitative and quantitative) to allow safe and wide treatment with 8-aminoquinolines.

SUBMITTER: Bancone G

PROVIDER: S-EPMC6343352 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Publications

Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion.

Bancone Germana G Menard Didier D Khim Nimol N Kim Saorin S Canier Lydie L Nguong Chea C Phommasone Koukeo K Mayxay Mayfong M Dittrich Sabine S Vongsouvath Malavanh M Fievet Nadine N Le Hesran Jean-Yves JY Briand Valerie V Keomany Sommay S Newton Paul N PN Gorsawun Gornpan G Tardy Kaelan K Chu Cindy S CS Rattanapalroj Orpreeya O Dong Le Thanh LT Quang Huynh Hong HH Tam-Uyen Nguyen N Thuy-Nhien Nguyen N Hien Tran Tinh TT Kalnoky Michael M Nosten Francois F

Malaria journal 20190123 1

<h4>Background</h4>Plasmodium vivax malaria elimination can only be achieved by the deployment of 8-aminoquinolines (primaquine and tafenoquine) in combination with ACT to kill both blood and liver-stage parasites. However, primaquine and the other 8-aminoquinolines cause dose-dependent haemolysis in subjects with G6PD deficiency, an X-linked disorder of red blood cells that is very common in populations living in tropical and subtropical areas. In order to inform safer use of 8-aminoquinolines ...[more]

PMID: 30674319

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Dataset Information

Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion.

Background

Methods

Results

Conclusions

Publications

Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion.

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