Discovery of SERPINA3 as a candidate urinary biomarker of lupus nephritis activity.

ABSTRACT:

Objectives

We used an unbiased proteomics approach to identify candidate urine biomarkers (CUBMs) predictive of LN chronicity and pursued their validation in a larger cohort.

Methods

In this cross-sectional pilot study, we selected urine collected at kidney biopsy from 20 children with varying levels of LN damage (discovery cohort) and performed proteomic analysis using isobaric tags for relative and absolute quantification (iTRAQ). We identified differentially excreted proteins based on degree of LN chronicity and sought to distinguish markers exhibiting different relative expression patterns using hierarchically clustered log10-normalized relative abundance data with linked and distinct functions by biological network analyses. For each CUBM, we performed specific ELISAs o

SUBMITTER: Turnier JL 

PROVIDER: S-EPMC6343468 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature