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Cytokine production in whole-blood cultures following immunization with an influenza vaccine.


ABSTRACT: A clinical trial of a quadrivalent split influenza vaccine was performed in the 2014/15 season. Sixty-four subjects aged 6 months to 18 years were enrolled in order to investigate the relationship between cellular and humoral immune responses. Subjects were categorized into two groups by measuring neutralizing antibodies: non-primed naïve/primed or seroconverted/non-seroconverted groups. Whole-blood cultures were stimulated with the H1N1 split antigen before immunization and one month after the first and second immunizations for subjects < 13 years and before and one month after the first dose for those ? 13 years in order to investigate cytokine production. Significant amounts of IL-2, IL-12, IL-13, MCP-1, MIP-1?, and TNF-? were detected from one month after the first dose in the naïve group. In addition to these cytokines, the production of IL-1?, IL-4, IL-6, IL-8, IL-10, IL-17, G-CSF, and IFN-? was enhanced one month after the second dose. No significant increase was noted in the primed group, except in the production of IL-10. In seroconverted subjects, the production of IL-2, IL-4, IL-8, IL-10, G-CSF, MCP-1, TNF-?, and IFN-? increased one month after the first dose, which was earlier than in the naïve group, whereas no significant cytokine response was noted in subjects without seroconversion. Subjects ? 13 years were primed and the production of G-CSF, IL-4, and IL-1? increased in subjects with seroconversion. Whole-blood cultures were also stimulated with the H3N2 split antigen and similar cytokine profiles were obtained. Many cytokines and chemokines, including inflammatory cytokines, were produced in seroconverted, but not non-seroconverted subjects.

SUBMITTER: Nakayama T 

PROVIDER: S-EPMC6343617 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Cytokine production in whole-blood cultures following immunization with an influenza vaccine.

Nakayama Tetsuo T   Kumagai Takuji T   Kashiwagi Yasuyo Y   Yoshii Hironori H   Honjo Kenta K   Kubota-Koketsu Ritsuko R   Okuno Yoshinobu Y   Suga Shigeru S  

Human vaccines & immunotherapeutics 20180828 12


A clinical trial of a quadrivalent split influenza vaccine was performed in the 2014/15 season. Sixty-four subjects aged 6 months to 18 years were enrolled in order to investigate the relationship between cellular and humoral immune responses. Subjects were categorized into two groups by measuring neutralizing antibodies: non-primed naïve/primed or seroconverted/non-seroconverted groups. Whole-blood cultures were stimulated with the H1N1 split antigen before immunization and one month after the  ...[more]

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