Complete Genome Sequence of Vibrio mediterranei 117-T6, a Potentially Pathogenic Bacterium Isolated from the Conchocelis of Pyropia spp.
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ABSTRACT:
SUBMITTER: Liu Q
PROVIDER: S-EPMC6346174 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Publications
Complete Genome Sequence of Vibrio mediterranei 117-T6, a Potentially Pathogenic Bacterium Isolated from the Conchocelis of Pyropia spp.
Liu Qiqin Q Xu Mengya M He Yingyun Y Tao Zhen Z Yang Rui R Chen Haimin H
Microbiology resource announcements 20190117 3
Vibrio mediterranei is a Gram-negative bacterium of the family Vibrionaceae. Vibrio mediterranei strain 117-T6 was pathogenic to Pyropia yezoensis, a red seaweed cultivated in China, by causing death to its conchocelis. Here, we report the complete genome sequence of Vibrio mediterranei 117-T6. ...[more]
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Project description:Vibrio mediterranei strain:117-T6 | isolate:Pyropia yezoensis conchocelis Genome sequencing and assembly
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Project description:BackgroundAcanthamoeba- bacteria interactions enable pathogenic bacteria to tolerate harsh conditions and lead to transmission to the susceptible host. The present study was aimed to address the presence of bacterial endosymbionts of Acanthamoeba isolated from recreational water sources of Tehran, Iran. To the best of our knowledge this is the first study regarding occurrence of bacteria in environmental Acanthamoeba spp. in Iran.MethodsA total of 75 samples of recreational water sources were collected. Samples were cultured on non- nutrient agar 1.5% plates. Positive Acanthamoeba spp. were axenically grown. DNA extraction and PCR reaction was performed using JDP1-2 primers. All positive samples of Acanthamoeba were examined for the presence of endosymbionts using staining and molecular methods. The PCR products were then sequenced in order to determine the genotypes of Acanthamoeba and bacteria genera.ResultsOut of 75 samples, 16 (21.3%) plates were positive for Acanthamoeba according to the morphological criteria. Molecular analysis revealed that Acanthamoeba belonged to T4 and T5 genotypes. Five isolates (35.7%) were positive for bacterial endosymbionts using staining method and PCR test. Sequencing of PCR products confirmed the presence of Pseudomonas aeruginosa and Agrobacterium tumefasiens.ConclusionThe presence of Acanthamoeba bearing pathogenic endosymbionts in water sources leads us to public health issues including improved sanitation and decontamination measures in recreational water sources in order to prevent amoebae-related infection. To the best of our knowledge this is the first report regarding the isolation of A. tumefasiens from Acanthamoeba in Iran and worldwide.
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Project description:Here, we report on the draft genome sequence of Vibrio mediterranei strain CyArs1, isolated from the marine sponge Cinachyrella sp. Genome annotation revealed multiple genomic features, including eukaryotic-like repeat protein- and multidrug resistance-encoding genes, potentially involved in symbiotic relationships with the sponge host.
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