Short-term intermittent PTH 1-34 administration and bone marrow blood vessel ossification in Mature and Middle-Aged C57BL/6 mice.

ABSTRACT: Intermittent parathyroid hormone (PTH) administration augments bone and progressive bone marrow blood vessel (BMBV) ossification occurs with advancing age. Since intermittent PTH administration augments bone, it may also serve to increase BMBV ossification. We assessed the influence of 5- and 10-days of intermittent PTH 1-34 administration on trabecular and cortical bone and BMBV ossification in mature (6-8 mon; n?=?30) and middle-aged (10-12 mon; n?=?30) male and female C57BL/6 mice. Mice were divided accordingly: control (CON) and 5-days (5dPTH) and 10-days (10dPTH) of PTH. Mice were given PBS (50??l) or PTH 1-34 (43??g/kg/d) for 5- and 10-consecutive days. Trabecular bone microarchitecture (i.e., BV/TV [%], Tb.Th [?m], Tb.N [/mm], and Tb.Sp [?m]) was assessed in the distal femoral metaphysis and cortical bone parameters (i.e., Ct.Th [?m] and CSMI [mm4]) at the femoral mid-shaft. BMBV ossification (i.e., ossified vessel volume [OsVV, %] and ossified vessel thickness [OsV.Th, ?m]) was assessed in the medullary cavity of the femoral shaft. All parameters were determined by ?CT. At this sample size, no gender-related differences were observed so female and male data were pooled. There were no main effects nor interactions for trabecular microarchitecture and Ct.Th. However, CSMI was larger (p?

SUBMITTER: Lee S 

PROVIDER: S-EPMC6348201 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature