Dataset Information

Biodegradation of naphthalene, BTEX, and aliphatic hydrocarbons by Paraburkholderia aromaticivorans BN5 isolated from petroleum-contaminated soil.

ABSTRACT: To isolate bacteria responsible for the biodegradation of naphthalene, BTEX (benzene, toluene, ethylbenzene, and o-, m-, and p-xylene), and aliphatic hydrocarbons in petroleum-contaminated soil, three enrichment cultures were established using soil extract as the medium supplemented with naphthalene, BTEX, or n-hexadecane. Community analyses showed that Paraburkholderia species were predominant in naphthalene and BTEX, but relatively minor in n-hexadecane. Paraburkholderia aromaticivorans BN5 was able to degrade naphthalene and all BTEX compounds, but not n-hexadecane. The genome of strain BN5 harbors genes encoding 29 monooxygenases including two alkane 1-monooxygenases and 54 dioxygenases, indicating that strain BN5 has versatile metabolic capabilities, for diverse organic compounds: the ability of strain BN5 to degrade short chain aliphatic hydrocarbons was verified experimentally. The biodegradation pathways of naphthalene and BTEX compounds were bioinformatically predicted and verified experimentally through the analysis of their metabolic intermediates. Some genomic features including the encoding of the biodegradation genes on a plasmid and the low sequence homologies of biodegradation-related genes suggest that biodegradation potentials of strain BN5 may have been acquired via horizontal gene transfers and/or gene duplication, resulting in enhanced ecological fitness by enabling strain BN5 to degrade all compounds including naphthalene, BTEX, and short aliphatic hydrocarbons in contaminated soil.

SUBMITTER: Lee Y

PROVIDER: S-EPMC6351602 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:Total petroleum hydrocarbons (TPHs), which are often found in soil, water, sediments, and air. These compounds are a type of pollutant that can have a serious negative impact on living things and human health. Soil washing method is a remediation technique used to remove contaminants from the soil. This process involves the use of water or other solvents to extract contaminants from the soil, followed by separation and disposal of the contaminated solution. This research engineered the effectiveness of soil washing method to remove TPHs from a genuine, sullied soil sample. After analyzing the physical and chemical properties of the soil, the Box-Benken Design (BBD) technique was used to optimize the variables that influence the process's effectiveness. A quadratic model was suggested based on the BBD design, correlation coefficients, and other factors. The minimum, maximum and mean removal of TPHs during the stages of the study were 63.5, 94.5 and 76.7%, respectively. The correlation between the variables was strong, as shown by the analysis of variance (ANOVA), F-value (1064.5) and P-value (0.0001), and the proposed model was highly significant. The most effective soil washing method (SWM) was obtained with pH 7.8, liquid to solid ratio 50:1, reaction time 52 min, surfactant concentration 7.9 mg kg-1, and three washings. A removal rate of 98.8% was accomplished for TPHs from the soil in this context. The kinetic results indicate that the kinetic of TPHs removal follows the first-order kinetics (R2 = 0.96). There was not a major difference in the process's efficiency based on temperature. The removal efficiency heightened from 0 to 150 rpm and then remained steady. Introducing air flow increased the rate of removal, and the combination of ultrasonic waves with the reaction environment increased the process efficiency and decreased the time for the process and the amount of times it needed to be washed. An analysis of the washed soil both physically and chemically revealed a substantial decrease in the concentration of other elements.

Project description:Phytoremediation of petroleum hydrocarbons in subarctic regions relies on the successful establishment of plants that stimulate petroleum-degrading microorganisms, which can be challenging due to the extreme climate, limited nutrients, and difficulties in maintaining sites in remote locations. A long-term phytoremediation experiment was initiated in Alaska in 1995 with the introduction of grasses and/or fertilizer to petroleum hydrocarbon (PHC)-contaminated soils that were subsequently left unmanaged. In 2011, the PHC concentrations were below detection limits in all soils tested and the originally planted grasses had been replaced by volunteer plant species that had colonized the site. Here, we sought to understand how the original treatments influenced the structure of prokaryotic communities associated with plant species that colonized the soils and to assess the interactions between the rhizospheric and endophytic communities of the colonizing vegetation 20 years after the experiment was established. Metataxonomic analysis performed using 16S rRNA gene sequencing revealed that the original type of contaminated soil and phytoremediation strategy influenced the structure of both rhizospheric and endophytic communities of colonizing plants, even 20 years after the treatments were applied and following the disappearance of the originally planted grasses. Our findings demonstrate that the choice of initial phytoremediation strategy drove the succession of microorganisms associated with the colonizing vegetation. The outcome of this study provides new insight into the establishment of plant-associated microbial communities during secondary succession of subarctic areas previously contaminated by PHCs and indicates that the strategies for restoring these ecosystems influence the plant-associated microbiota in the long term. IMPORTANCE Subarctic ecosystems provide key services to local communities, yet they are threatened by pollution caused by spills and disposal of petroleum waste. Finding solutions for the remediation and restoration of subarctic soils is valuable for reasons related to human and ecosystem health, as well as environmental justice. This study provides novel insight into the long-term succession of soil and plant-associated microbiota in subarctic soils that had been historically contaminated with different sources of PHCs and subjected to distinct phytoremediation strategies. We provide evidence that even after the successful removal of PHCs and the occurrence of secondary succession, the fingerprint of the original source of contamination and the initial choice of remediation strategy can be detected as a microbial legacy in the rhizosphere, roots, and shoots of volunteer vegetation even 2 decades after the contamination had occurred. Such information needs to be borne in mind when designing and applying restoration approaches for PHC-contaminated soils in subarctic ecosystems.

Dataset Information

Biodegradation of naphthalene, BTEX, and aliphatic hydrocarbons by Paraburkholderia aromaticivorans BN5 isolated from petroleum-contaminated soil.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets