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Identification of the antioxidant defense genes which may provide enhanced salt tolerance in Oryza sativa L.

ABSTRACT: Antioxidative mechanisms are important to protect cells from the hazardous effects of reactive oxygen species (ROS). Salt stress is one of the environmental stress factors that leads to accumulation of ROS at toxic levels. In this study, we analyzed the responses of two rice (Oryza sativa L.) cultivars against NaCl stress at enzymatic and transcriptional levels. In 14 day-old-seedlings, different antioxidant enzyme activities were observed. These findings were also supported by transcriptional analyses of the responsible genes. According to the results, Cyt-APX, CAT A, Cyt-GR1 and proline metabolism-related genes were differentially expressed between two rice varieties under different salt concentrations. Their regulational differences cause different salt sensitivities of the varieties. By this study, we provided an insight into understanding of the correlation between antioxidant defence genes and ROS enzymes under salt stress.

SUBMITTER: Celik O

PROVIDER: S-EPMC6352531 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Identification of the antioxidant defense genes which may provide enhanced salt tolerance in <i>Oryza sativa</i> L.

Çelik Özge Ö Çakır Bilgin Candar BC Atak Çimen Ç

Physiology and molecular biology of plants : an international journal of functional plant biology 20181116 1

Antioxidative mechanisms are important to protect cells from the hazardous effects of reactive oxygen species (ROS). Salt stress is one of the environmental stress factors that leads to accumulation of ROS at toxic levels. In this study, we analyzed the responses of two rice (<i>Oryza sativa</i> L.) cultivars against NaCl stress at enzymatic and transcriptional levels. In 14 day-old-seedlings, different antioxidant enzyme activities were observed. These findings were also supported by transcript ...[more]

PMID: 30804632

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Project description:BACKGROUND AND AIMS:Salt stress leads to attenuated growth and productivity in rice. Transcription factors like heat shock factors (HSFs) represent central regulators of stress adaptation. Heat shock factors of the classes A and B are well established as regulators of thermal and non-thermal stress responses in plants; however, the role of class C HSFs is unknown. Here we characterized the function of the OsHsfC1b (Os01g53220) transcription factor from rice. METHODOLOGY:We analysed the expression of OsHsfC1b in the rice japonica cultivars Dongjin and Nipponbare exposed to salt stress as well as after mannitol, abscisic acid (ABA) and H(2)O(2) treatment. For functional characterization of OsHsfC1b, we analysed the physiological response of a T-DNA insertion line (hsfc1b) and two artificial micro-RNA (amiRNA) knock-down lines to salt, mannitol and ABA treatment. In addition, we quantified the expression of small Heat Shock Protein (sHSP) genes and those related to signalling and ion homeostasis by quantitative real-time polymerase chain reaction in roots exposed to salt. The subcellular localization of OsHsfC1b protein fused to green fluorescent protein (GFP) was determined in Arabidopsis mesophyll cell protoplasts. PRINCIPAL RESULTS:Expression of OsHsfC1b was induced by salt, mannitol and ABA, but not by H(2)O(2). Impaired function of OsHsfC1b in the hsfc1b mutant and the amiRNA lines led to decreased salt and osmotic stress tolerance, increased sensitivity to ABA, and temporal misregulation of salt-responsive genes involved in signalling and ion homeostasis. Furthermore, sHSP genes showed enhanced expression in knock-down plants under salt stress. We observed retarded growth of hsfc1b and knock-down lines in comparison with control plants under non-stress conditions. Transient expression of OsHsfC1b fused to GFP in protoplasts revealed nuclear localization of the transcription factor. CONCLUSIONS:OsHsfC1b plays a role in ABA-mediated salt stress tolerance in rice. Furthermore, OsHsfC1b is involved in the response to osmotic stress and is required for plant growth under non-stress conditions.

Project description:BackgroundHigh Salinity is a major environmental stress influencing growth and development of rice. Comparative proteomic analysis of hybrid rice shoot proteins from Shanyou 10 seedlings, a salt-tolerant hybrid variety, and Liangyoupeijiu seedlings, a salt-sensitive hybrid variety, was performed to identify new components involved in salt-stress signaling.ResultsPhenotypic analysis of one protein that was upregulated during salt-induced stress, cyclophilin 2 (OsCYP2), indicated that OsCYP2 transgenic rice seedlings had better tolerance to salt stress than did wild-type seedlings. Interestingly, wild-type seedlings exhibited a marked reduction in maximal photochemical efficiency under salt stress, whereas no such change was observed for OsCYP2-transgenic seedlings. OsCYP2-transgenic seedlings had lower levels of lipid peroxidation products and higher activities of antioxidant enzymes than wild-type seedlings. Spatiotemporal expression analysis of OsCYP2 showed that it could be induced by salt stress in both Shanyou 10 and Liangyoupeijiu seedlings, but Shanyou 10 seedlings showed higher OsCYP2 expression levels. Moreover, circadian rhythm expression of OsCYP2 in Shanyou 10 seedlings occurred earlier than in Liangyoupeijiu seedlings. Treatment with PEG, heat, or ABA induced OsCYP2 expression in Shanyou 10 seedlings but inhibited its expression in Liangyoupeijiu seedlings. Cold stress inhibited OsCYP2 expression in Shanyou 10 and Liangyoupeijiu seedlings. In addition, OsCYP2 was strongly expressed in shoots but rarely in roots in two rice hybrid varieties.ConclusionsTogether, these data suggest that OsCYP2 may act as a key regulator that controls ROS level by modulating activities of antioxidant enzymes at translation level. OsCYP2 expression is not only induced by salt stress, but also regulated by circadian rhythm. Moreover, OsCYP2 is also likely to act as a key component that is involved in signal pathways of other types of stresses-PEG, heat, cold, or ABA.

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Identification of the antioxidant defense genes which may provide enhanced salt tolerance in Oryza sativa L.

Publications

Identification of the antioxidant defense genes which may provide enhanced salt tolerance in <i>Oryza sativa</i> L.

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