Ontology highlight
ABSTRACT:
SUBMITTER: Maruyama S
PROVIDER: S-EPMC6353620 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Maruyama Shintaro S Suzuki Kano K Imamura Motonori M Sasaki Hikaru H Matsunami Hideyuki H Mizutani Kenji K Saito Yasuko Y Imai Fabiana L FL Ishizuka-Katsura Yoshiko Y Kimura-Someya Tomomi T Shirouzu Mikako M Uchihashi Takayuki T Ando Toshio T Yamato Ichiro I Murata Takeshi T
Science advances 20190130 1
V<sub>1</sub>-ATPase is an ATP-driven rotary motor that is composed of a ring-shaped A<sub>3</sub>B<sub>3</sub> complex and a central DF shaft. The nucleotide-free A<sub>3</sub>B<sub>3</sub> complex of <i>Enterococcus hirae</i>, composed of three identical A<sub>1</sub>B<sub>1</sub> heterodimers, showed a unique asymmetrical structure, probably due to the strong binding of the N-terminal barrel domain, which forms a crown structure. Here, we mutated the barrel region to weaken the crown, and per ...[more]