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Optimization of Subcritical Water Extraction (SWE) of Lipid and Eicosapentaenoic Acid (EPA) from Nannochloropsis gaditana.

ABSTRACT: Microalgae are a promising source of omega-3. The purpose of this study was to extract lipid with a relatively high content of eicosapentaenoic acid (EPA) from Nannochloropsis gaditana using subcritical water extraction (SWE). The effects of different temperatures (156.1-273.9°C), extraction times (6.6-23.4 minutes), and biomass loadings (33-117?g algae/L) on the extraction yield were studied. From the optimization study using central composite design (CCD), quadratic models generated for lipid yield and EPA composition were considered to be significant models (p < 0.05). The predictive equations were also formed for lipid yield and EPA composition. The predicted optimum lipid yield and EPA composition at 236.54°C, 13.95 minutes, and 60.50?g algae/L were 18.278?wt% of total biomass and 14.036?wt% of total fatty acid methyl ester (FAME), respectively.

SUBMITTER: Ho BCH

PROVIDER: S-EPMC6354137 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Optimization of Subcritical Water Extraction (SWE) of Lipid and Eicosapentaenoic Acid (EPA) from <i>Nannochloropsis gaditana</i>.

Ho Bernard Chon Han BCH Kamal Siti Mazlina Mustapa SMM Danquah Michael K MK Harun Razif R

BioMed research international 20181227

Microalgae are a promising source of omega-3. The purpose of this study was to extract lipid with a relatively high content of eicosapentaenoic acid (EPA) from <i>Nannochloropsis gaditana</i> using subcritical water extraction (SWE). The effects of different temperatures (156.1-273.9°C), extraction times (6.6-23.4 minutes), and biomass loadings (33-117 g algae/L) on the extraction yield were studied. From the optimization study using central composite design (CCD), quadratic models generated for ...[more]

PMID: 30775380

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