Mapping of ?/? T cells reveals V?2+ T cells resistance to senescence.

ABSTRACT:

Background

Immune adaptation with aging is a major of health outcomes. Studies in humans have mainly focus on ?? T cells while ?? T cells have been neglected despite their role in immunosurveillance. We investigated the impact of aging on ?? T cell subsets phenotypes, functions, senescence and their molecular response to stress.

Methods

Peripheral blood of young and old donors in Singapore have been used to assess the phenotype, functional capacity, proliferation capacity and gene expression of the various ?? T cell subsets. Peripheral blood mononuclear cells from apheresis cones and young donors have been used to characterize the telomere length, epigenetics profile and DNA damage response of the various ?? T cell subsets phenotype.

Findings

Our data shows that peripheral V?2+ phenotype, functional capacity (cytokines, cytotoxicity, proliferation) and gene expression profile are specific when compared against all other ?? and ?? T cells in aging. Hallmarks of senescence including telomere length, epigenetic profile and DNA damage response of V?2+ also differs against all other ?? and ?? T cells.

Interpretation

Our results highlight the differential impact of lifelong stress on ?? T cells subsets, and highlight possible mechanisms that enable V?2+ to be resistant to cellular aging. The new findings reinforce the concept that V?2+ have an "innate-like" behavior and are more resilient to the environment as compared to "adaptive-like" V?1+ T cells.

SUBMITTER: Xu W 

PROVIDER: S-EPMC6354624 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature