Oxygen uptake in complexes related to [NiFeS]- and [NiFeSe]-hydrogenase active sites.

ABSTRACT: A biomimetic study for S/Se oxygenation in Ni(?-EPh)(?-SN₂)Fe, (E = S or Se; SN₂ = Me-diazacycloheptane-CH₂CH₂S); Fe = (?⁵-C₅H₅)Fe^II(CO) complexes related to the oxygen-damaged active sites of [NiFeS]/[NiFeSe]-H₂ases is described. Mono- and di-oxygenates (major and minor species, respectively) of the chalcogens result from exposure of the heterobimetallics to O₂; one was isolated and structurally characterized to have Ni-O-Se_Ph-Fe-S connectivity within a 5-membered ring. A compositionally analogous mono-oxy species was implicated by ?(CO) IR spectroscopy to be the corresponding Ni-O-S_Ph-Fe-S complex; treatment with O-abstraction agents such as P(o-tolyl)₃ or PMe₃ remediated the O damage. Computational studies (DFT) found that the lowest energy isomers of mono-oxygen derivatives of Ni(?-EPh)(?-SN₂)Fe complexes were those with O attachment to Ni rather than Fe, a result consonant with experimental findings, but at odds with oxygenates found in oxygen-damaged [NiFeS]/[NiFeSe]-H₂ase structures. A computer-generated model based on substituting ^-SMe for the N-CH₂CH₂S^- sulfur donor of the N₂S suggested that constraint within the chelate hindered O-atom uptake at that sulfur site.

SUBMITTER: Yang X 

PROVIDER: S-EPMC6354737 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature