Nitrogen gas produces less behavioural and neurophysiological excitation than carbon dioxide in mice undergoing euthanasia.
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ABSTRACT:
SUBMITTER: Detotto C
PROVIDER: S-EPMC6354991 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
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Nitrogen gas produces less behavioural and neurophysiological excitation than carbon dioxide in mice undergoing euthanasia.
Detotto Carlotta C Isler Sarah S Wehrle Martin M Vyssotski Alexei L AL Bettschart-Wolfensberger Regula R Gent Thomas C TC
PloS one 20190131 1
Carbon dioxide (CO2) is one of the most commonly used gas euthanasia agents in mice, despite reports of aversion and nociception. Inert gases such as nitrogen (N2) may be a viable alternative to carbon dioxide. Here we compared behavioural and electrophysiological reactions to CO2 or N2 at either slow fill or rapid fill in C57Bl/6 mice undergoing gas euthanasia. We found that mice euthanised with CO2 increased locomotor activity compared to baseline, whereas mice exposed to N2 decreased locomoti ...[more]
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