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Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris.


ABSTRACT: The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (Oryzias latipes) (md) gonadotropins Fsh? (mdFsh?), Lh? (mdLh?), Fsh?? (mdFsh??), and Lh?? (mdLh??) generated by Pichia pastoris, as well as data on a validation of produced antibodies against Fsh? and Lh? by Western blot analysis. Furthermore, the article includes data on Fsh and Lh protein levels in male medaka pituitaries using recombinant mdFsh?? and mdLh?? within enzyme-linked immunosorbent assays (ELISAs), in which protein amounts were analyzed related to body weight and age of the fish. This dataset is associated with the research article entitled "Medaka Follicle-stimulating hormone (Fsh) and Luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression" (Burow et al., in press).

SUBMITTER: Burow S 

PROVIDER: S-EPMC6355963 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Data on Western blot and ELISA analysis of medaka (<i>Oryzias latipes</i>) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with <i>Pichia pastoris</i>.

Burow Susann S   Fontaine Romain R   von Krogh Kristine K   Mayer Ian I   Nourizadeh-Lillabadi Rasoul R   Hollander-Cohen Lian L   Cohen Yaron Y   Shpilman Michal M   Levavi-Sivan Berta B   Weltzien Finn-Arne FA  

Data in brief 20190119


The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (<i>Oryzias latipes</i>) (md) gonadotropins Fshβ (mdFshβ), Lhβ (mdLhβ), Fshβα (mdFshβα), and Lhβα (mdLhβα) generated by <i>Pichia pastoris,</i> as well as data on a validation of produced antibodies against Fshβ and Lhβ by Western blot analysis. Furthermore, the article includes data on F  ...[more]

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