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Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections.


ABSTRACT: A lytic bacteriophage PHB01 specific for Pasteurella multocida type D was isolated from the sewage water collected from a pig farm. This phage had the typical morphology of the family Podoviridae, order Caudovirales, presenting an isometric polyhedral head and a short noncontractile tail. PHB01 was able to infect most of the non-toxigenic P. multocida type D strains tested, but not toxigenic type D strains and those belonging to other capsular types. Phage PHB01, the first lytic phage specific for P. multocida type D sequenced thus far, presents a 37,287-bp double-stranded DNA genome with a 223-bp terminal redundancy. The PHB01 genome showed the highest homology with that of PHB02, a lytic phage specific for P. multocida type A. Phylogenetic analysis showed that PHB01 and PHB02 were composed of a genus that was close to the T7-virus genus. In vivo tests using mouse models showed that the administration of PHB01 was safe to the mice and had a good effect on treating the mice infected with different P. multocida type D strains including virulent strain HN05. These findings suggest that PHB01 has a potential use in therapy against infections caused by P. multocida type D.

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6356340 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Isolation of a T7-Like Lytic <i>Pasteurella</i> Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against <i>Pasteurella multocida</i> Infections.

Chen Yibao Y   Guo Guanghao G   Sun Erchao E   Song Jiaoyang J   Yang Lan L   Zhu Lili L   Liang Wan W   Hua Lin L   Peng Zhong Z   Tang Xibiao X   Chen Huanchun H   Wu Bin B  

Viruses 20190121 1


A lytic bacteriophage PHB01 specific for <i>Pasteurella multocida</i> type D was isolated from the sewage water collected from a pig farm. This phage had the typical morphology of the family <i>Podoviridae</i>, order <i>Caudovirales</i>, presenting an isometric polyhedral head and a short noncontractile tail. PHB01 was able to infect most of the non-toxigenic <i>P. multocida</i> type D strains tested, but not toxigenic type D strains and those belonging to other capsular types. Phage PHB01, the  ...[more]

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