Unknown

Dataset Information

0

Immunostimulatory Effects of Chitooligosaccharides on RAW 264.7 Mouse Macrophages via Regulation of the MAPK and PI3K/Akt Signaling Pathways.

ABSTRACT: Chitooligosaccharides (COS), the hydrolyzed products of chitin and chitosan, can be obtained by various methods. In this study, water-soluble COS were prepared from ?- and ?-chitosan by microwave-assisted degradation and their immunostimulatory effects were investigated in RAW 264.7 macrophages. The results indicated that ?-COS were more active than ?-COS in promoting the production of nitric oxide (NO) and cytokines, such as tumor necrosis factor-? (TNF-?) and interleukin 6 (IL-6). Quantitative real-time reverse transcription polymerase chain reaction and Western blotting indicated that COS also enhanced the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-?. Further analyses demonstrated that COS induced the phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38, p85 and Akt, and the nuclear translocation of p65, indicating that they are able to activate the mitogen-activated protein kinases (MAPKs) and phosphoinositide 3-kinases (PI3K)/Akt signaling pathways dependent on nuclear factor (NF)-?B activation. In conclusion, COS activate RAW 264.7 cells via the MAPK and PI3K/Akt signaling pathways and are potential novel immune potentiators.

SUBMITTER: Yang Y 

PROVIDER: S-EPMC6357175 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Immunostimulatory Effects of Chitooligosaccharides on RAW 264.7 Mouse Macrophages via Regulation of the MAPK and PI3K/Akt Signaling Pathways.

Yang Yue Y   Xing Ronge R   Liu Song S   Qin Yukun Y   Li Kecheng K   Yu Huahua H   Li Pengcheng P  

Marine drugs 20190108 1

Chitooligosaccharides (COS), the hydrolyzed products of chitin and chitosan, can be obtained by various methods. In this study, water-soluble COS were prepared from α- and β-chitosan by microwave-assisted degradation and their immunostimulatory effects were investigated in RAW 264.7 macrophages. The results indicated that α-COS were more active than β-COS in promoting the production of nitric oxide (NO) and cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6). Quantitative  ...[more]

Similar Datasets

Unknown Scandoside Exerts Anti-Inflammatory Effect Via Suppressing NF-?B and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages.
| S-EPMC5855679 | biostudies-literature
Unknown C60 fullerene localization and membrane interactions in RAW 264.7 immortalized mouse macrophages.
| S-EPMC4761875 | biostudies-literature
Transcriptomics LPS stimulation of Mouse (RAW 264.7) macrophages
2016-02-25 | GSE76562 | GEO
Transcriptomics LPS stimulation of Mouse (RAW 264.7) macrophages
2016-02-25 | E-GEOD-76562 | biostudies-arrayexpress
Unknown Anti-Inflammatory Effect of Baicalein on Polyinosinic?Polycytidylic Acid-Induced RAW 264.7 Mouse Macrophages.
| S-EPMC5977217 | biostudies-literature
Unknown The immunostimulatory effect of indole-6-carboxaldehyde isolated from <i>Sargassum thunbergii</i> <b>(Mertens) Kuntze</b> in RAW 264.7 macrophages.
| S-EPMC7473310 | biostudies-literature
Unknown Bromo-honaucin A inhibits osteoclastogenic differentiation in RAW 264.7 cells via Akt and ERK signaling pathways.
| S-EPMC4684412 | biostudies-literature
Unknown Anti-Inflammatory Effect of Quercetin on RAW 264.7 Mouse Macrophages Induced with Polyinosinic-Polycytidylic Acid.
| S-EPMC6273652 | biostudies-literature
Unknown Anti-Inflammatory Effect of Wogonin on RAW 264.7 Mouse Macrophages Induced with Polyinosinic-Polycytidylic Acid.
| S-EPMC6273246 | biostudies-literature
Unknown 5-Methoxyl Aesculetin Abrogates Lipopolysaccharide-Induced Inflammation by Suppressing MAPK and AP-1 Pathways in RAW 264.7 Cells.
| S-EPMC4813178 | biostudies-literature