Dataset Information

High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells.

ABSTRACT: Distant metastasis is the main cause of death for non-small cell lung cancer (NSCLC) patients, with the bone as a more frequent metastatic site. The expression of various chemokines and their receptors may contribute to the predilection of organ specific metastasis. In this study, we demonstrated that CC chemokine ligand 7 (CCL7) and its receptors CCR1, CCR2 and CCR3 were up-regulated markedly in lung cancer bone metastasis. In addition, CCL7 promoted migration and invasion of lung cancer cells in a dose-dependent pattern but played an insignificant role in cell proliferation mainly through CCR3. Finally, we identified a cohort of critical downstream genes of CCL7 related to cancer metastasis using Illumina deep mRNA sequencing. These findings may help better understand the molecular aspects of bone metastasis in lung cancer, and prefigure a potential clinical value for the prevention of bone recurrences.

SUBMITTER: Han S

PROVIDER: S-EPMC6357330 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells.

Han Shuai S Wang Ting T Chen Yuanming Y Han Zhitao Z Guo Lihan L Wu Zhipeng Z Yan Wangjun W Wei Haifeng H Liu Tielong T Zhao Jian J Zhou Wang W Yang Xinghai X Xiao Jianru J

American journal of translational research 20190115 1

Distant metastasis is the main cause of death for non-small cell lung cancer (NSCLC) patients, with the bone as a more frequent metastatic site. The expression of various chemokines and their receptors may contribute to the predilection of organ specific metastasis. In this study, we demonstrated that CC chemokine ligand 7 (CCL7) and its receptors CCR1, CCR2 and CCR3 were up-regulated markedly in lung cancer bone metastasis. In addition, CCL7 promoted migration and invasion of lung cancer cells ...[more]

PMID: 30788000

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Project description:Calponin 3 (CNN3) is known to serve a role in certain types of cancer, such as gastric cancer and colorectal cancer. The present study investigated the clinical significance of CNN3 in non-small cell lung cancer (NSCLC) by evaluating its expression profile and relationship with disease prognosis using the Gene Expression Omnibus repository, Gene Expression Profiling Interactive Analysis 2 (GEPIA2) and Kaplan-Meier plotter analysis. CNN3 mRNA expression was measured using reverse transcription-quantitative PCR, while the protein expression level was measured using western blot analysis. Cell proliferation, cell cycle and apoptosis, and migration and invasion were analyzed using MTS assay, flow cytometry and Transwell assays, respectively. These results revealed that CNN3 mRNA expression was downregulated in NSCLC tissues compared with that in normal tissues. Additionally, CNN3 expression had a high diagnostic value based on the GSE2514 dataset and the data from The Cancer Genome Atlas and the Genotype Tissue Expression database, whereas it had a low diagnostic value based on the GSE10072 dataset. Furthermore, CNN3 expression was associated with survival in patients with lung adenocarcinoma (LUAD), whereas it was not associated with survival in patients with lung squamous cell carcinoma (LUSC) according to the Kaplan-Meier plotter results. According to the data from GEPIA2, and the GSE72094, GSE41271 and GSE31210 datasets, CNN3 expression was not associated with the prognosis of patients with LUAD and LUSC. The mRNA and protein expression levels of CNN3 were lower in two NSCLC cell lines (A549 and SK-MES-1) than in a human bronchial epithelial cell line (BEAS-2B). CNN3 overexpression suppressed cell proliferation, migration and invasion, induced G1-phase arrest, promoted apoptosis and suppressed PI3K/AKT signaling pathway activation in the NSCLC cell lines, whereas CNN3 overexpression had no effect on cell morphology. In conclusion, CNN3 suppressed the proliferation and metastasis of NSCLC cells by downregulating the PI3K/AKT signaling pathway, making it a potential therapeutic target in this disease.

Dataset Information

High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells.

Publications

High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells.

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