Dataset Information

Genome Sequence of Enterococcus faecalis NVIT04, Isolated from Nasonia vitripennis.

ABSTRACT: Enterococcus faecalis is a Gram-positive, lactic acid-producing coccus which can be found as a member of the gut microbiome in many animal species and is a potential pathogen in humans. Here, we describe the genome sequence of an E. faecalis strain isolated from the gut microbiome of the hymenopteran model Nasonia vitripennis.

SUBMITTER: Wang GH

PROVIDER: S-EPMC6357635 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Genome Sequence of Enterococcus faecalis NVIT04, Isolated from Nasonia vitripennis.

Wang Guan-Hong GH Brucker Robert M RM

Microbiology resource announcements 20190131 5

Enterococcus faecalis is a Gram-positive, lactic acid-producing coccus which can be found as a member of the gut microbiome in many animal species and is a potential pathogen in humans. Here, we describe the genome sequence of an E. faecalis strain isolated from the gut microbiome of the hymenopteran model Nasonia vitripennis. ...[more]

PMID: 30714029

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Project description:The emergence of antimicrobial-resistant, livestock-associated Enterococcus faecalis represents a public health concern. Here, we report the isolation, molecular detection of virulence and antimicrobial resistance determinants, in addition to the phylogenetic analyses of 20 Enterococcus species using whole genome sequencing analysis of 15 Enterococcus faecalis strains including six strains of three novel sequence types, three Enterococcus faecium and two Enterococcus durans. All strains were isolated from food chain animals in South Africa. Enterococcus strains were isolated on bile aesculin azide agar, followed by identification using MALDI-TOF MS analysis. Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method. The genomic DNA of the isolates was extracted and sequencing was performed using the Illumina MiSeq platform. Sequence reads were trimmed and de novo assembled. The assembled contigs were analyzed for antimicrobial resistance genes and chromosomal mutations, extra-chromosomal plasmids, and multi-locus sequence type (MLST). Multidrug antimicrobial resistance genes conferring resistance to aminoglycosides (ant(6)-Ia, aph(3')-IIIa, sat4, and spw), lincosamides (lnu(B), lsa(A), and lsa(E)), macrolides (erm(B)), trimethoprim (dfrG) and tetracyclines (tet(L) and tet(M)) were identified. Plasmid replicons were detected in seven E. faecalis and three E. faecium isolates. The sequence type (ST) of each isolate was determined using the Enterococcus PubMLST database. Ten STs were identified in the collection, three of which (ST1240, ST1241, and ST1242) have not been previously reported and are described in the present study for the first time. To compare the sequenced strains to other previously sequenced E. faecalis strains, assembled sequences of E. faecalis from livestock were downloaded from the PubMLST database. Core genome-based phylogenetic analysis was performed using ParSNP. The detection of multiple drug-resistance in Enterococcus including E. faecalis and E. faecium highlights the significance of genomic surveillance to monitor the spread of antimicrobial resistance in food chain animals. In addition, the genome sequences of Enterococcus strains reported in the present study will serve as a reference point for future molecular epidemiological studies of livestock-associated and antibiotic-resistant E. faecalis in Africa. In addition, this study enables the in-depth analysis of E. faecalis genomic structure, as well as provides valuable information on the phenotypic and genotypic antimicrobial resistance, and the pathogenesis of livestock-associated E. faecalis and E. faecium.

Dataset Information

Genome Sequence of Enterococcus faecalis NVIT04, Isolated from Nasonia vitripennis.

Publications

Genome Sequence of Enterococcus faecalis NVIT04, Isolated from Nasonia vitripennis.

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