Dataset Information

Draft Genome Sequence of Erwinia psidii, Causal Agent of Bacterial Blight of Guava (Psidium guava) and Dieback of Eucalypt (Eucalyptus spp.).

ABSTRACT: Here, we present a draft genome sequence of the type strain IBSBF 435 of Erwinia psidii (Enterobacteriaceae), a phytopathogen that causes bacterial blight on guava (Psidium guava) and dieback and wilt on eucalypt (Eucalyptus spp.), both of which are important emerging diseases.

SUBMITTER: Hermenegildo PDS

PROVIDER: S-EPMC6357642 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Draft Genome Sequence of Erwinia psidii, Causal Agent of Bacterial Blight of Guava (Psidium guava) and Dieback of Eucalypt (Eucalyptus spp.).

Hermenegildo Pollyane da Silva PDS Santos Samuel A SA Guimarães Lúcio M S LMS Pereira Isadora C IC Vidigal Pedro M P PMP Badel Jorge L JL Alfenas-Zerbini Poliane P Mafia Reginaldo G RG Ferreira Marisa A S V MASV Alfenas Acelino Couto AC

Microbiology resource announcements 20190131 5

Here, we present a draft genome sequence of the type strain IBSBF 435 of Erwinia psidii (Enterobacteriaceae), a phytopathogen that causes bacterial blight on guava (Psidium guava) and dieback and wilt on eucalypt (Eucalyptus spp.), both of which are important emerging diseases. ...[more]

PMID: 30714036

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Project description:The Brazilian guava processing industry generates 5.5 M Mg guava waste year(-1) that could be recycled sustainably in guava agro-ecosystems as slow-release fertilizer. Our objectives were to elaborate nutrient budgets and to diagnose soil, foliar, and fruit nutrient balances in guava orchards fertilized with guava waste. We hypothesized that (1) guava waste are balanced fertilizer sources that can sustain crop yield and soil nutrient stocks, and (2) guava agroecosystems remain productive within narrow ranges of nutrient balances. A 6-year experiment was conducted in 8-year old guava orchard applying 0-9-18-27-36 Mg ha(-1) guava waste (dry mass basis) and the locally recommended mineral fertilization. Nutrient budgets were compiled as balance sheets. Foliar and fruit nutrient balances were computed as isometric log ratios to avoid data redundancy or resonance due to nutrient interactions and the closure to measurement unit. The N, P, and several other nutrients were applied in excess of crop removal while K was in deficit whatever the guava waste treatment. The foliar diagnostic accuracy reached 93% using isometric log ratios and knn classification, generating reliable foliar nutrient and concentration ranges at high yield level. The plant mined the soil K reserves without any significant effect on fruit yield and foliar nutrient balances involving K. High guava productivity can be reached at lower soil test K and P values than thought before. Parsimonious dosage of fresh guava waste should be supplemented with mineral K fertilizers to recycle guava waste sustainably in guava agroecosystems. Brazilian growers can benefit from this research by lowering soil test P and K threshold values to avoid over-fertilization and using fresh guava waste supplemented with mineral fertilizers, especially K. Because yield was negatively correlated with fruit acidity and Brix index, balanced plant nutrition and fertilization diagnosis will have to consider not only fruit yield targets but also fruit quality to meet requirements for guava processing.

Project description:Understanding how environmental variables influence biofilm formation becomes relevant for managing Vibrio biofilm-related infections in shrimp production. Therefore, we evaluated the impact of temperature, time, and initial inoculum in the biofilm development of these two Vibrio species using a multifactorial experimental design. Planktonic growth inhibition and inhibition/eradication of Vibrio biofilms, more exactly V. parahaemolyticus (VP87 and VP275) and V. cholerae (VC112) isolated from shrimp farms were evaluated by Eucalyptus and Guava aqueous leaf extracts and compared to tetracycline and ceftriaxone. Preliminary results showed that the best growth conditions of biofilm development for V. parahaemolyticus were 24 h and 24°C (p <0.001), while V. cholerae biofilms were 72 h and 30°C (p <0.001). Multivariate linear regression ANOVA was applied using colony-forming unit (CFU) counting assays as a reference, and R-squared values were applied as goodness-of-fit measurements for biofilm analysis. Then, both plant extracts were analyzed with HPLC using double online detection by diode array detector (DAD) and mass spectrometry (MS) for the evaluation of their chemical composition, where the main identified compounds for Eucalyptus extract were cypellogin A, cypellogin B, and cypellocarpin C, while guavinoside A, B, and C compounds were the main compounds for Guava extract. For planktonic growth inhibition, Eucalyptus extract showed its maximum effect at 200 μg/mL with an inhibition of 75% (p < 0.0001) against all Vibrio strains, while Guava extract exhibited its maximum inhibition at 1600 μg/mL with an inhibition of 70% (p < 0.0001). Both biofilm inhibition and eradication assays were performed by the two conditions (24 h at 24°C and 72 h at 30°C) on Vibrio strains according to desirability analysis. Regarding 24 h at 24°C, differences were observed in the CFU counting between antibiotics and plant extracts, where both plant extracts demonstrated a higher reduction of viable cells when compared with both antibiotics at 8x, 16x, and 32x MIC values (Eucalyptus extract: 1600, 3200, and 6400 μg/mL; while Guava extract: 12800, 25600, and 52000 μg/mL). Concerning 72 h at 30°C, results showed a less notorious biomass inhibition by Guava leaf extract and tetracycline. However, Eucalyptus extract significantly reduced the total number of viable cells within Vibrio biofilms from 2x to 32x MIC values (400-6400 μg/mL) when compared to the same MIC values of ceftriaxone (5-80 μg/mL), which was not able to reduce viable cells. Eucalyptus extract demonstrated similar results at both growth conditions, showing an average inhibition of approximately 80% at 400 μg/mL concentration for all Vibrio isolates (p < 0.0001). Moreover, eradication biofilm assays demonstrated significant eradication against all Vibrio strains at both growth conditions, but biofilm eradication values were substantially lower. Both extract plants demonstrated a higher reduction of viable cells when compared with both antibiotics at 8x, 16x, and 32x MIC values at both growth sets, where Eucalyptus extract at 800 μg/mL reduced 70% of biomass and 90% of viable cells for all Vibrio strains (p < 0.0001). Overall results suggested a viable alternative against vibriosis in the shrimp industry in Ecuador.

Dataset Information

Draft Genome Sequence of Erwinia psidii, Causal Agent of Bacterial Blight of Guava (Psidium guava) and Dieback of Eucalypt (Eucalyptus spp.).

Publications

Draft Genome Sequence of Erwinia psidii, Causal Agent of Bacterial Blight of Guava (<i>Psidium guava</i>) and Dieback of Eucalypt (<i>Eucalyptus</i> spp.).

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