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ABSTRACT:
SUBMITTER: Lopez-Rodriguez E
PROVIDER: S-EPMC6358299 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Lopez-Rodriguez Elena E Roldan Nuria N Garcia-Alvarez Begoña B Pérez-Gil Jesús J
Journal of lipid research 20181121 2
TLC has traditionally been used to analyze lipids isolated from membrane complexes. Here, we describe a method based on the combination of TLC and SDS-PAGE to qualitatively analyze the protein/lipid profile of membrane complexes such as those of lung surfactant. For this purpose, native lung surfactant was applied onto a silica TLC plate in the form of an aqueous suspension, preserving not only hydrophilic proteins associated with lipids but also native protein-lipid interactions. Using native m ...[more]