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Electrospun Polycaprolactone Fibrous Membranes Containing Ag, TiO? and Na?Ti?O₁₃ Particles for Potential Use in Bone Regeneration.

ABSTRACT: Biocompatible and biodegradable membrane treatments for regeneration of bone are nowadays a promising solution in the medical field. Bioresorbable polymers are extensively used in membrane elaboration, where polycaprolactone (PCL) is used as base polymer. The goal of this work was to improve electrospun membranes' biocompatibility and antibacterial properties by adding micro- and nanoparticles such as Ag, TiO? and Na?Ti?O₁₃. Micro/nanofiber morphologies of the obtained membranes were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, differential scanning calorimetry, scanning electron microscopy, energy-dispersive X-ray spectroscopy and a tensile test. Also, for this study optical microscopy was used to observe DAPI-stained cells. Membranes of the different systems were electrospun to an average diameter of 1.02?1.76 ?m. To evaluate the biological properties, cell viability was studied by growing NIH/3T3 cells on the microfibers. PCL/TiO? strength was enhanced from 0.6 MPa to 6.3 MPa in comparison with PCL without particles. Antibacterial activity was observed in PCL/TiO? and PCL/Na?Ti?O₁₃ electrospun membranes using Staphylococcus aureus bacteria. Bioactivity of the membranes was confirmed with simulated body fluid (SBF) treatment. From this study, the ceramic particles TiO? and Na?Ti?O₁₃, combined with a PCL matrix with micro/nanoparticles, enhanced cell proliferation, adhesion and antibacterial properties. The electrospun composite with Na?Ti?O₁₃ can be considered viable for tissue regenerative processes.

SUBMITTER: Ramirez-Cedillo E

PROVIDER: S-EPMC6359384 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Electrospun Polycaprolactone Fibrous Membranes Containing Ag, TiO₂ and Na₂Ti₆O<sub>13</sub> Particles for Potential Use in Bone Regeneration.

Ramírez-Cedillo Erick E Ortega-Lara Wendy W Rocha-Pizaña María R MR Rocha-Pizaña María R MR Gutierrez-Uribe Janet A JA Elías-Zúñiga Alex A Rodríguez Ciro A CA

Membranes 20190110 1

Biocompatible and biodegradable membrane treatments for regeneration of bone are nowadays a promising solution in the medical field. Bioresorbable polymers are extensively used in membrane elaboration, where polycaprolactone (PCL) is used as base polymer. The goal of this work was to improve electrospun membranes' biocompatibility and antibacterial properties by adding micro- and nanoparticles such as Ag, TiO₂ and Na₂Ti₆O<sub>13</sub>. Micro/nanofiber morphologies of the obtained membranes were ...[more]

PMID: 30634630

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Project description:This article reports on a study of the mineralisation behaviour of CaCO3 deposited on electrospun poly(ε-caprolactone) (PCL) scaffolds preliminarily treated with low-temperature plasma. This work was aimed at developing an approach that improves the wettability and permeability of PCL scaffolds in order to obtain a superior composite coated with highly porous CaCO3, which is a prerequisite for biomedical scaffolds used for drug delivery. Since PCL is a synthetic polymer that lacks functional groups, plasma processing of PCL scaffolds in O2, NH3, and Ar atmospheres enables introduction of highly reactive chemical groups, which influence the interaction between organic and inorganic phases and govern the nucleation, crystal growth, particle morphology, and phase composition of the CaCO3 coating. Our studies showed that the plasma treatment induced the formation of O- and N-containing polar functional groups on the scaffold surface, which caused an increase in the PCL surface hydrophilicity. Mineralisation of the PCL scaffolds was performed by inducing precipitation of CaCO3 particles on the surface of polymer fibres from a mixture of CaCl2- and Na2CO3-saturated solutions. The presence of highly porous vaterite and nonporous calcite crystal phases in the obtained coating was established. Our findings confirmed that preferential growth of the vaterite phase occurred in the O2-plasma-treated PCL scaffold and that the coating formed on this scaffold was smoother and more homogenous than those formed on the untreated PCL scaffold and the Ar- and NH3-plasma-treated PCL scaffolds. A more detailed three-dimensional assessment of the penetration depth of CaCO3 into the PCL scaffold was performed by high-resolution micro-computed tomography. The assessment revealed that O2-plasma treatment of the PCL scaffold caused CaCO3 to nucleate and precipitate much deeper inside the porous structure. From our findings, we conclude that O2-plasma treatment is preferable for PCL scaffold surface modification from the viewpoint of use of the PCL/CaCO3 composite as a drug delivery platform for tissue engineering.

Dataset Information

Electrospun Polycaprolactone Fibrous Membranes Containing Ag, TiO? and Na?Ti?O₁₃ Particles for Potential Use in Bone Regeneration.

Publications

Electrospun Polycaprolactone Fibrous Membranes Containing Ag, TiO₂ and Na₂Ti₆O<sub>13</sub> Particles for Potential Use in Bone Regeneration.

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Dataset Information

Electrospun Polycaprolactone Fibrous Membranes Containing Ag, TiO? and Na?Ti?O13 Particles for Potential Use in Bone Regeneration.

Publications

Electrospun Polycaprolactone Fibrous Membranes Containing Ag, TiO₂ and Na₂Ti₆O<sub>13</sub> Particles for Potential Use in Bone Regeneration.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Electrospun Polycaprolactone Fibrous Membranes Containing Ag, TiO? and Na?Ti?O₁₃ Particles for Potential Use in Bone Regeneration.