Dataset Information

Effect of superparamagnetic nanoparticles coated with various electric charges on ?-synuclein and ?-amyloid proteins fibrillation process.

ABSTRACT:

Background

Most of nanoparticles are nontoxic and have high absorption capability. Therefore, nanoparticles binding can effectively restrain fibrillation of ?-amyloid and ?-synuclein proteins and eventually prevent the toxicity of pathogenesis peptide of Alzheimer. Super paramagnetic iron oxide nanoparticles (SPIONs) contain iron oxide core which can be connected to a special part through magnetic coating.

Materials and methods

In this study, the effect of SPIONs with different charges was simultaneously examined on the fibrillation of both ?-amyloid and ?-synuclein proteins by applying Thioflavin-T assay.

Results

According to the results of the investigation on amyloid-fibrillation mechanism in both ?-amyloids and ?-synucleins, it was revealed that negatively-charged nanoparticles encoded to -COOH by dextran-coating were able to have a considerable absorption decrease from 17,000-12,000 after 320 minutes delay to lag phase and decrease in binding level of thioflavin-T particles to ?-sheets.

Conclusion

The different concentrations of these nanoparticles and special coating of each particle had an effect on the kinetics of ?-amyloid and ?-synuclein fibrillations.

SUBMITTER: Javdani N

PROVIDER: S-EPMC6361412 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Effect of superparamagnetic nanoparticles coated with various electric charges on α-synuclein and β-amyloid proteins fibrillation process.

Javdani Negin N Rahpeyma Sayyed Shahryar SS Ghasemi Younes Y Raheb Jamshid J

International journal of nanomedicine 20190123

<h4>Background</h4>Most of nanoparticles are nontoxic and have high absorption capability. Therefore, nanoparticles binding can effectively restrain fibrillation of β-amyloid and α-synuclein proteins and eventually prevent the toxicity of pathogenesis peptide of Alzheimer. Super paramagnetic iron oxide nanoparticles (SPIONs) contain iron oxide core which can be connected to a special part through magnetic coating.<h4>Materials and methods</h4>In this study, the effect of SPIONs with different ch ...[more]

PMID: 30774334

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