Dataset Information
Effect of superparamagnetic nanoparticles coated with various electric charges on ?-synuclein and ?-amyloid proteins fibrillation process.
Ontology highlight
ABSTRACT: Background
Most of nanoparticles are nontoxic and have high absorption capability. Therefore, nanoparticles binding can effectively restrain fibrillation of ?-amyloid and ?-synuclein proteins and eventually prevent the toxicity of pathogenesis peptide of Alzheimer. Super paramagnetic iron oxide nanoparticles (SPIONs) contain iron oxide core which can be connected to a special part through magnetic coating.Materials and methods
In this study, the effect of SPIONs with different charges was simultaneously examined on the fibrillation of both ?-amyloid and ?-synuclein proteins by applying Thioflavin-T assay.Results
According to the results of the investigation on amyloid-fibrillation mechanism in both ?-amyloids and ?-synucleins, it was revealed that negatively-charged nanoparticles encoded to -COOH by dextran-coating were able to have a considerable absorption decrease from 17,000-12,000 after 320 minutes delay to lag phase and decrease in binding level of thioflavin-T particles to ?-sheets.Conclusion
The different concentrations of these nanoparticles and special coating of each particle had an effect on the kinetics of ?-amyloid and ?-synuclein fibrillations.
SUBMITTER: Javdani N
PROVIDER: S-EPMC6361412 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Effect of superparamagnetic nanoparticles coated with various electric charges on α-synuclein and β-amyloid proteins fibrillation process.
International journal of nanomedicine 20190123
<h4>Background</h4>Most of nanoparticles are nontoxic and have high absorption capability. Therefore, nanoparticles binding can effectively restrain fibrillation of β-amyloid and α-synuclein proteins and eventually prevent the toxicity of pathogenesis peptide of Alzheimer. Super paramagnetic iron oxide nanoparticles (SPIONs) contain iron oxide core which can be connected to a special part through magnetic coating.<h4>Materials and methods</h4>In this study, the effect of SPIONs with different ch ...[more]
