Unknown

Dataset Information

0

Live single cell mass spectrometry reveals cancer-specific metabolic profiles of circulating tumor cells.

ABSTRACT: Recently, there has been increased attention on the analysis of circulating tumor cells (CTCs), also known as liquid biopsy, owing to its potential benefits in cancer diagnosis and treatment. Circulating tumor cells are released from primary tumor lesions into the blood stream and eventually metastasize to distant body organs. However, a major hurdle with CTC analysis is their natural scarcity. Existing methods lack sensitivity, specificity, or reproducibility required in CTC characterization and detection. Here, we report untargeted molecular profiling of single CTCs obtained from gastric cancer and colorectal cancer patients, using live single cell mass spectrometry integrated with microfluidics-based cell enrichment techniques. Using this approach, we showed the difference in the metabolomic profile between CTCs originating from different cancer groups. Moreover, potential biomarkers were putatively annotated to be specific to each cancer type.

SUBMITTER: Abouleila Y 

PROVIDER: S-EPMC6361580 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Recently, there has been increased attention on the analysis of circulating tumor cells (CTCs), also known as liquid biopsy, owing to its potential benefits in cancer diagnosis and treatment. Circulating tumor cells are released from primary tumor lesions into the blood stream and eventually metastasize to distant body organs. However, a major hurdle with CTC analysis is their natural scarcity. Existing methods lack sensitivity, specificity, or reproducibility required in CTC characterization an  ...[more]

Similar Datasets

Unknown Mass Spectrometry Imaging-Based Single-Cell Lipidomics Profiles Metabolic Signatures of Heart Failure.
| S-EPMC10076023 | biostudies-literature
Unknown Imaging mass spectrometry reveals unique protein profiles during embryo implantation.
| S-EPMC2453082 | biostudies-other
Unknown Microscale Mass Spectrometry Analysis of Extracellular Metabolites in Live Multicellular Tumor Spheroids.
| S-EPMC5912160 | biostudies-other
Unknown Metabolomic Studies of Live Single Cancer Stem Cells Using Mass Spectrometry.
| S-EPMC6582952 | biostudies-literature
Unknown A Multiplexed Cytokeratin Analysis Using Targeted Mass Spectrometry Reveals Specific Profiles in Cancer-Related Pleural Effusions.
| S-EPMC4954941 | biostudies-literature
Unknown In Situ Microprobe Single-Cell Capillary Electrophoresis Mass Spectrometry: Metabolic Reorganization in Single Differentiating Cells in the Live Vertebrate (Xenopus laevis) Embryo.
| S-EPMC5706767 | biostudies-literature
Unknown Profiling 26,000 Aplysia californica neurons by single cell mass spectrometry reveals neuronal populations with distinct neuropeptide profiles.
| S-EPMC9396074 | biostudies-literature
Unknown Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging.
| S-EPMC8642449 | biostudies-literature
Unknown An end-to-end deep learning method for mass spectrometry data analysis to reveal disease-specific metabolic profiles.
| S-EPMC11335749 | biostudies-literature
Unknown Quantification of Drug Molecules in Live Single Cells Using the Single-Probe Mass Spectrometry Technique.
| S-EPMC6677389 | biostudies-literature