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Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson's Disease Patients.


ABSTRACT: We report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson's disease (PD) patients' peripheral tissues. We have previously shown that Saa-1 and -2 (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients' skin, using whole-genome RNA sequencing. In the current study, we characterized the gene and protein expression profiles of skin and blood samples from patients with confirmed PD diagnosis and age/sex matched controls. qRT-PCR analysis of PD skin demonstrated downregulation of Saa-1 and -2 genes in PD patients. However, the lowered amount of protein could not be visualized using immunohistochemistry, due to low quantity of SAA (Serum Amyloid Alpha, protein) in skin. Saa-1 and -2 expression levels in whole blood were below detection threshold based on RNA sequencing, however significantly lowered protein levels of SAA1/2 in PD patients' serum were shown with ELISA, implying that SAA is secreted into the blood. These results show that SAA is differentially expressed in the peripheral tissues of PD patients.

SUBMITTER: Kurvits L 

PROVIDER: S-EPMC6361740 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson's Disease Patients.

Kurvits Lille L   Reimann Ene E   Kadastik-Eerme Liis L   Truu Laura L   Kingo Külli K   Erm Triin T   Kõks Sulev S   Taba Pille P   Planken Anu A  

Frontiers in neuroscience 20190129


We report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson's disease (PD) patients' peripheral tissues. We have previously shown that <i>Saa-1</i> and <i>-2</i> (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients' skin, using whole-genome RNA sequencing. In the current study, we characterized the gene and protein expression profiles of skin and blood samples from patients with confirmed PD diagnosis and age/sex matched  ...[more]

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