Ontology highlight
ABSTRACT:
SUBMITTER: Lee EEL
PROVIDER: S-EPMC6363413 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Lee Elizabeth E L EEL Bezanilla Francisco F
The Journal of general physiology 20190103 2
<i>Xenopus laevis</i> oocytes are a widely used model system because of their capacity to translate exogenous mRNA, but their high intrinsic background fluorescence is a disadvantage for fluorescence recordings. Here, we developed two distinct methods for improving fluorescence recordings from oocytes. One was a pharmacological method in which a small-molecule salt-inducible kinase inhibitor was co-injected with the mRNA of interest to stimulate melanin production. We interrogated the oocytes us ...[more]