Dataset Information

Paternal leakage and mtDNA heteroplasmy in Rhipicephalus spp. ticks.

ABSTRACT: Paternal leakage of mitochondrial DNA (mtDNA) and heteroplasmy have been recently described in several animal species. In arthropods, by searching in the Scopus database, we found only 23 documented cases of paternal leakage. Therefore, although arthropods represent a large fraction of animal biodiversity, this phenomenon has been investigated only in a paucity of species in this phylum, thus preventing a reliable estimate of its frequency. Here, we investigated the occurrence of paternal leakage and mtDNA heteroplasmy in ticks belonging to one of the most significant tick species complexes, the so-called Rhipicephalus sanguineus sensu lato. By developing a multiplex allele-specific PCR assay targeting a fragment of the 12S rRNA ribosomal region of the mtDNA, we showed the occurrence of paternal leakage and mtDNA heteroplasmy in R. sanguineus s.l. ticks originated from experimental crosses, as well as in individuals collected from the field. Our results add a new evidence of paternal leakage in arthropods and document for the first time this phenomenon in ticks. Furthermore, they suggest the importance of using allele-specific assays when searching for paternal leakage and/or heteroplasmy, as standard sequencing methods may fail to detect the rare mtDNA molecules.

SUBMITTER: Mastrantonio V

PROVIDER: S-EPMC6365633 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Paternal leakage and mtDNA heteroplasmy in Rhipicephalus spp. ticks.

Mastrantonio Valentina V Latrofa Maria Stefania MS Porretta Daniele D Lia Riccardo Paolo RP Parisi Antonio A Iatta Roberta R Dantas-Torres Filipe F Otranto Domenico D Urbanelli Sandra S

Scientific reports 20190206 1

Paternal leakage of mitochondrial DNA (mtDNA) and heteroplasmy have been recently described in several animal species. In arthropods, by searching in the Scopus database, we found only 23 documented cases of paternal leakage. Therefore, although arthropods represent a large fraction of animal biodiversity, this phenomenon has been investigated only in a paucity of species in this phylum, thus preventing a reliable estimate of its frequency. Here, we investigated the occurrence of paternal leakag ...[more]

PMID: 30728407

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Dataset Information

Paternal leakage and mtDNA heteroplasmy in Rhipicephalus spp. ticks.

Publications

Paternal leakage and mtDNA heteroplasmy in Rhipicephalus spp. ticks.

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