Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction.
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ABSTRACT: BACKGROUND:Acute myocardial infarction (AMI) is characterized by an inflammatory process in which T cell plays a key role. However, the profile of immune microenvironment in AMI is still uncertain. High-throughput sequencing of T cell receptor (TCR) provides deep insight into monitoring the immune microenvironment. METHODS:30 patients with AMI were enrolled and 30 healthy individuals were recruited as controls. Flow cytometer were used to analyze the distribution of ?? T cells and their CD69 expression from peripheral leukomonocytes. TCR? repertoire library was amplified by two-round multiplex PCR and detected by next-generation sequencing (NGS). RESULTS:The percentage of ?? T cells in AMI patients were significantly restricted than those in healthy controls, while the highly activated ?? T cells along with distinguishing usage of variable (V), diversity (D) and joining (J) gene segments were also found in AMI patients. In addition, AMI induced a significantly restricted CDR3 amino acid (AA) diversity and remarkably reconstituted TCR immune repertoires. Finally, we identified several AMI-associated tendency of CDR3 AAs expression after AMI. CONCLUSIONS:Our work suggests that the aberrant ?? T cells distribution and activation may associated with the pathogenesis of AMI and demonstrates a reconstitution of TCR? immune repertoire after AMI.
SUBMITTER: Li D
PROVIDER: S-EPMC6366076 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
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