Dataset Information

Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize.

ABSTRACT: The anionic peroxidases play an important role in a variety of plant physiological processes. We characterized and isolated the Zmap promoter (PZmap) at the 5' flanking region in order to better understand the regulatory mechanisms of Zmap gene expression. A series of PZmap deletion derivatives, termed a1 -a6, at positions -1694, -1394, -1138, -784, -527 and -221 from the translation start site were blended to the ?-glucuronidase reporter gene. Agrobacterium-mediated transformation method was used to study each deletion construct in tobaccos. Sequence analysis showed that several cis-acting elements (MYB binding site, Box-II, a TGACG-element, a CGTCA-element and a low temperature responsive element) were located within the promoter. Deletion analysis suggested the sequence between -1,694 and -1394bp may contain cis-elements associated with GUS up regulation. The MYB binding site (-757) might act as a negative drought-responsive element. There might be repressor elements located in the region (-1,694 to -1394bp) to repress Zmap expression under 4°C. The characterized promoter would be an ideal candidate for genetic engineering for improving the resistance of maize to different stressors.

SUBMITTER: Jin B

PROVIDER: S-EPMC6368321 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize.

Jin Bo B Sheng Zunlai Z Muhammad Ishfaq I Chen Jianqing J Yang Hongliang H

PloS one 20190208 2

The anionic peroxidases play an important role in a variety of plant physiological processes. We characterized and isolated the Zmap promoter (PZmap) at the 5' flanking region in order to better understand the regulatory mechanisms of Zmap gene expression. A series of PZmap deletion derivatives, termed a1 -a6, at positions -1694, -1394, -1138, -784, -527 and -221 from the translation start site were blended to the β-glucuronidase reporter gene. Agrobacterium-mediated transformation method was us ...[more]

PMID: 30735543

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Dataset Information

Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize.

Publications

Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize.

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