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Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology.

ABSTRACT: Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads were detected. Subsequently, a specific molecular test and immunohistochemistry were used to confirm the presence of CDV RNA and antigen, respectively, in tissues. This study supports the use of the NGS in veterinary clinical practice with potential advantages in terms of rapidity and broad-range of molecular diagnosis.

SUBMITTER: Peserico A

PROVIDER: S-EPMC6368598 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology.

Peserico Alessia A Marcacci Maurilia M Malatesta Daniela D Di Domenico Marco M Pratelli Annamaria A Mangone Iolanda I D'Alterio Nicola N Pizzurro Federica F Cirone Francesco F Zaccaria Guendalina G Cammà Cesare C Lorusso Alessio A

Scientific reports 20190208 1

Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads were detected. Subsequently, a specific molecular test and immunohistochemistry were used to confirm the ...[more]

PMID: 30737428

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