Dataset Information

Kartogenin Enhances Collagen Organization and Mechanical Strength of the Repaired Enthesis in a Murine Model of Rotator Cuff Repair.

ABSTRACT: PURPOSE:To investigate the use of kartogenin (KGN) in augmenting healing of the repaired enthesis after rotator cuff repair in a murine model. METHODS:Seventy-two C57BL/6 wild-type mice underwent unilateral detachment and transosseous repair of the supraspinatus tendon augmented with either fibrin sealant (control group; n = 36) or fibrin sealant containing 100 ?mol/L of KGN (experimental group; n = 36) applied at the repair site. Postoperatively, mice were allowed free cage activity without immobilization. Mice were humanely killed at 2 and 4 weeks postoperatively. Repair site integrity was evaluated histologically through fibrocartilage formation and collagen fiber organization and biomechanically through load-to-failure testing of the supraspinatus tendon-bone construct. RESULTS:At 2 weeks, no differences were noted in percent area of fibrocartilage, collagen organization, or ultimate strength between groups. At 4 weeks, superior collagen fiber organization (based on collagen birefringence [17.3 ± 2.0 vs 7.0 ± 6.5 integrated density/?m2; P < .01]) and higher ultimate failure loads (3.5 ± 0.6 N vs 2.3 ± 1.1 N; P = .04) were seen in the KGN group. The percent area of fibrocartilage (13.2 ± 8.4% vs 4.4 ± 5.4%; P = .04) was higher in the control group compared with the KGN group. CONCLUSIONS:Rotator cuff repair augmentation with KGN improved the collagen fiber organization and biomechanical strength of the tendon-bone interface at 4 weeks in a murine model. CLINICAL RELEVANCE:These findings have implications for improving the structural integrity of the repaired enthesis and potentially reducing the retear rate after rotator cuff repair, which can ultimately lead to improvements in clinical outcomes.

SUBMITTER: Wang D

PROVIDER: S-EPMC6371391 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Kartogenin Enhances Collagen Organization and Mechanical Strength of the Repaired Enthesis in a Murine Model of Rotator Cuff Repair.

Wang Dean D Tan Hongbo H Lebaschi Amir H AH Nakagawa Yusuke Y Wada Susumu S Donnelly Patrick E PE Ying Liang L Deng Xiang-Hua XH Rodeo Scott A SA

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association 20180720 9

<h4>Purpose</h4>To investigate the use of kartogenin (KGN) in augmenting healing of the repaired enthesis after rotator cuff repair in a murine model.<h4>Methods</h4>Seventy-two C57BL/6 wild-type mice underwent unilateral detachment and transosseous repair of the supraspinatus tendon augmented with either fibrin sealant (control group; n = 36) or fibrin sealant containing 100 μmol/L of KGN (experimental group; n = 36) applied at the repair site. Postoperatively, mice were allowed free cage activ ...[more]

PMID: 30037570

Similar Datasets

Project description:Myosteatosis is the pathological accumulation of lipid that occurs in conjunction with atrophy and fibrosis following skeletal muscle injury or disease. Little is known about the mechanisms by which lipid accumulates in myosteatosis, but many studies have demonstrated the degree of lipid infiltration negatively correlates with muscle function and regeneration. Our goal was to identify biochemical pathways that lead to muscle dysfunction and lipid accumulation in injured rotator cuff muscles, a model that demonstrates severe myosteatosis. Adult rats were subjected to a massive tear to the rotator cuff musculature. After a period of either 0 (healthy control), 10, 30, or 60 days, muscles were prepared for RNA sequencing, shotgun lipidomics, metabolomics, biochemical measures, electron microscopy, and muscle fiber contractility. Following rotator cuff injury, there was a decrease in muscle fiber specific force production that was lowest at 30d. There was a dramatic time dependent increase in triacylglyceride content. Interestingly, genes related to not only triacylglyceride synthesis, but also lipid oxidation were largely downregulated over time. Using bioinformatics techniques, we identified that biochemical pathways related to mitochondrial dysfunction and reactive oxygen species were considerably increased in muscles with myosteatosis. Long chain acyl-carnitines and L-carnitine, precursors to beta-oxidation, were depleted following rotator cuff tear. Electron micrographs showed injured muscles displayed large lipid droplets within mitochondria at early time points, and an accumulation of peripheral segment mitochondria at all time points. Several markers of oxidative stress were elevated following rotator cuff tear. The results from this study suggest that the accumulation of lipid in myosteatosis is not a result of canonical lipid synthesis, but occurs due to decreased lipid oxidation in mitochondria. A failure in lipid utilization by mitochondria would ultimately cause an accumulation of lipid even in the absence of increased synthesis. Further study will identify whether this process is required for the onset of myosteatosis.

Project description:PurposeTo assess the mechanical properties, tendon resistance to suture cutout, and knot size of a broad variety of high-strength sutures and tapes available for arthroscopic rotator cuff repair.MethodsNine different types of high-strength sutures and tapes for arthroscopic rotator cuff repair were studied: 6 were tapes (FiberTape, Hi-Fi Tape, Permatape, SutureTape, UltraTape, and XBraid TT), and 3 were sutures (Dynacord, FiberWire, and Ultrabraid). First, mechanical tensile testing of suture loops (n = 6) was performed. Second, the suture material was passed through an intact human cadaveric rotator cuff tendon (supraspinatus or infraspinatus), and cyclic as well as load-to-failure testing was performed, 8 times for each suture or tape. Statistical analysis of groups (tapes vs sutures) and between each suture and each tape was performed.ResultsMaterial testing revealed significant differences with superior mechanical properties of tapes compared with sutures regarding load for 3 mm of displacement (201 N vs 84 N, P < .0001), displacement at 200 N (3.6 mm vs 6.6 mm, P < .0001), stiffness (46 N/mm vs 25 N/mm, P < .0001), and ultimate load to failure (509 N vs 288 N, P < .0001). FiberTape showed the highest ultimate load to failure (805.5 ± 36.1 N), the highest load necessary for 3 mm of displacement (376.2 ± 19.1 N), and the lowest displacement at a 200-N load (2 ± 0.1 mm). Permatape had the highest stiffness (58.5 ± 5.3 N/mm). FiberTape had the highest knot height (9.5 ± 1.3 mm) and knot width (3.8 ± 0.7 mm) of a suture loop with 7 square knots. The typical failure mode in the cadaveric study part was tendon cut through.ConclusionsBiomechanical in vitro testing showed that high-strength suture tapes compared with regular high-strength sutures have significantly better mechanical properties in both dry-laboratory testing and human cadaveric rotator cuff tendon pullout testing. FiberTape outperformed the other tapes and the sutures used in this analysis. Nonetheless, differences in tendon testing appeared to be less substantial than in dry-laboratory testing, and FiberTape had the highest knot height and width.Clinical relevanceFiberTape revealed the most favorable biomechanical performance in dry-laboratory and rotator cuff tendon testing. It may provide the best repair strength in vivo; however, it also has the largest knot size.

Dataset Information

Kartogenin Enhances Collagen Organization and Mechanical Strength of the Repaired Enthesis in a Murine Model of Rotator Cuff Repair.

Publications

Kartogenin Enhances Collagen Organization and Mechanical Strength of the Repaired Enthesis in a Murine Model of Rotator Cuff Repair.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets