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Structural basis for chemically-induced homodimerization of a single domain antibody.

ABSTRACT: Chemically-induced dimerization (CID) systems are essential tools to interrogate and control biological systems. AcVHH is a single domain antibody homo-dimerizing upon caffeine binding. AcVHH has a strong potential for clinical applications through caffeine-mediated in vivo control of therapeutic gene networks. Here we provide the structural basis for caffeine-induced homo-dimerization of acVHH.

SUBMITTER: Lesne J

PROVIDER: S-EPMC6372657 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Structural basis for chemically-induced homodimerization of a single domain antibody.

Lesne Jean J Chang Hung-Ju HJ De Visch Angelique A Paloni Matteo M Barthe Philippe P Guichou Jean-François JF Mayonove Pauline P Barducci Alessandro A Labesse Gilles G Bonnet Jerome J Cohen-Gonsaud Martin M

Scientific reports 20190212 1

Chemically-induced dimerization (CID) systems are essential tools to interrogate and control biological systems. AcVHH is a single domain antibody homo-dimerizing upon caffeine binding. AcVHH has a strong potential for clinical applications through caffeine-mediated in vivo control of therapeutic gene networks. Here we provide the structural basis for caffeine-induced homo-dimerization of acVHH. ...[more]

PMID: 30755682

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