Identification of molecular biomarkers for the diagnosis of gastric cancer and lymph-node metastasis.

ABSTRACT:

Background and objective

Biomarkers are important tools for prompt diagnosis of cancer. This study aimed to identify reliable biomarkers for clinical applications in the diagnosis of gastric cancer and lymph-node (LN) metastasis.

Methods

Between 1 December 2014 and 31 December 2015, we prospectively collected samples of gastric-cancer tissues, corresponding matched-pair normal gastric mucosa, and their peri-gastric metastatic and non-metastatic LNs to identify quantitatively reliable genes using quantitative real-time polymerase chain reaction. Relative quantity (RQ) was used to calculate the mRNA expression levels of our target genes. Statistics were calculated using one-way analysis of variance (ANOVA) and Tukey's multiple comparison test. Analytical graphs were plotted using GraphPad Prism.

Results

Of nine assessed genes, the mRNA levels of inhibin beta A (INHBA) and secreted phosphoprotein 1 (SPP1) were most consistently highly expressed in tumor tissues by 15.4- and 15.6-fold, respectively, as compared with normal tissues (P?SPP1 mRNA expression between metastatic and non-metastatic LNs (P?=?0.470). In contrast, the INHBA mRNA level was up-regulated 4.1-fold in metastatic LNs (P?P?=?0.003), M0 and M1 (P?=?0.043) and different histological variants (intestinal type vs diffuse type, P?=?0.019).

Conclusions

Our results showed that INHBA was the most optimally reliable biomarker for diagnosing gastric cancer and LN metastasis.

SUBMITTER: Seeruttun SR 

PROVIDER: S-EPMC6375354 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature