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Real-time imaging of senescence in tumors with DNA damage.

ABSTRACT: Detection of cellular senescence is important not only in the study of senescence in various biological systems, but also in various practical applications such as image-guided surgical removal of senescent cells, as well as the monitoring of drug-responsiveness during cancer therapies. Due to the lack of suitable imaging probes for senescence detection, particularly in living subjects, we have developed an activatable near-infrared (NIR) molecular probe with far-red excitation, NIR emission, and high "turn-on" ratio upon senescence-associated β-galactosidase (SABG) activation. We present here the first successful demonstration of NIR imaging of DNA damage-induced senescence both in vitro and in human tumor xenograft models.

SUBMITTER: Wang Y

PROVIDER: S-EPMC6375927 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Real-time imaging of senescence in tumors with DNA damage.

Wang Ying Y Liu Jun J Ma Xiaowei X Cui Chao C Deenik Philip R PR Henderson Paul K P PKP Sigler Ashton L AL Cui Lina L

Scientific reports 20190214 1

Detection of cellular senescence is important not only in the study of senescence in various biological systems, but also in various practical applications such as image-guided surgical removal of senescent cells, as well as the monitoring of drug-responsiveness during cancer therapies. Due to the lack of suitable imaging probes for senescence detection, particularly in living subjects, we have developed an activatable near-infrared (NIR) molecular probe with far-red excitation, NIR emission, an ...[more]

PMID: 30765819

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