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A monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for flubendiamide detection.


ABSTRACT: Flubendiamide (FD), the first commercial phthalic acid diamide that targets insect ryanodine receptor (RyRs), has played an important role in pest management. With its extensive worldwide application, a rapid and convenient method to detect its existence in the environment is necessary. In this study, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to analyse FD residue on environmental and food samples. The established icELISA showed a half maximal inhibition concentration (IC50) of 17.25?µg?L-1, with a working range of 4.06-103.59?µg?L-1 for FD, and showed no cross-reactivity with chlorantraniliprole, cyantraniliprole, and several FD analogues. Average FD recoveries from spinach, tap water, and soil samples were 89.3-112.3%, 93.0-102.1%, and 86.9-97.6%, respectively. Meanwhile, FD detection results of icELISA were compared with those of ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The comparable results verified that icELISA was suitable for rapid detection of FD residue in environmental and agricultural samples.

SUBMITTER: Li Q 

PROVIDER: S-EPMC6376021 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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A monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for flubendiamide detection.

Li Qibo Q   Cui Yongliang Y   Liao Min M   Feng Tong T   Tan Guiyu G   Wang Baomin B   Liu Shangzhong S  

Scientific reports 20190214 1


Flubendiamide (FD), the first commercial phthalic acid diamide that targets insect ryanodine receptor (RyRs), has played an important role in pest management. With its extensive worldwide application, a rapid and convenient method to detect its existence in the environment is necessary. In this study, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to analyse FD residue on environmental and food samples. The established icELISA showed a half maximal inhibition c  ...[more]

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