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Comparison of individual, group and environmental sampling strategies to conduct influenza surveillance in pigs.


ABSTRACT: BACKGROUND:Influenza A virus (IAV) is an important pathogen in pigs that affects productivity and has important public health implications because of its zoonotic nature. Surveillance is central to the control of influenza, however, detection of IAV infections can be challenging in endemically infected herds with low prevalence of infection. METHODS:In groups of suckling (18-21?days of age) and growing (35-45?days of age) pigs, we compared various sampling approaches to detect, isolate and sequence IAV using individual (nasal swabs, nasal wipes and oropharyngeal swabs), group (oral fluids, surface wipes and sow udder skin wipes) and environmental (airborne particles deposited on surfaces and air samples) sampling approaches. All samples were tested by IAV rRT-PCR and a subset was used for virus isolation and direct sequencing. RESULTS:In general, environmental and group samples resulted in higher odd ratios (range?=?3.87-16.5, p-value?

SUBMITTER: Garrido-Mantilla J 

PROVIDER: S-EPMC6376652 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Comparison of individual, group and environmental sampling strategies to conduct influenza surveillance in pigs.

Garrido-Mantilla Jorge J   Alvarez Julio J   Culhane Marie M   Nirmala Jayaveeramuthu J   Cano Jean Paul JP   Torremorell Montserrat M  

BMC veterinary research 20190214 1


<h4>Background</h4>Influenza A virus (IAV) is an important pathogen in pigs that affects productivity and has important public health implications because of its zoonotic nature. Surveillance is central to the control of influenza, however, detection of IAV infections can be challenging in endemically infected herds with low prevalence of infection.<h4>Methods</h4>In groups of suckling (18-21 days of age) and growing (35-45 days of age) pigs, we compared various sampling approaches to detect, is  ...[more]

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