Ontology highlight
ABSTRACT:
SUBMITTER: Andrews NP
PROVIDER: S-EPMC6377228 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Andrews Nicolas P NP Boeckman Justin X JX Manning Colleen F CF Nguyen Joe T JT Bechtold Hannah H Dumitras Camelia C Gong Belvin B Nguyen Kimberly K van der List Deborah D Murray Karl D KD Engebrecht JoAnne J Trimmer James S JS
eLife 20190122
Generating recombinant monoclonal antibodies (R-mAbs) from mAb-producing hybridomas offers numerous advantages that increase the effectiveness, reproducibility, and transparent reporting of research. We report here the generation of a novel resource in the form of a library of recombinant R-mAbs validated for neuroscience research. We cloned immunoglobulin G (IgG) variable domains from cryopreserved hybridoma cells and input them into an integrated pipeline for expression and validation of funct ...[more]