Dataset Information

Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.

ABSTRACT:

Objective

To examine the expression of hypoxia-inducible factor-1? (HIF-1?), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.

Methods

TfR1 and HIF-1? were detected by western blot. Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding.

Results

There was no difference in the expression of TfR1 and HIF-1? between groups. Lectin blot analysis pointed out an overexpression of galactose ?1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.

Conclusion

The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins. These findings may be associated with the low expression of placental TfR1 in women with preeclampsia.

SUBMITTER: Gomez-Gutierrez AM

PROVIDER: S-EPMC6378037 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.

Gómez-Gutiérrez Alejandra María AM Parra-Sosa Beatriz Elena BE Bueno-Sánchez Julio Cesar JC

Journal of pregnancy 20190203

<h4>Objective</h4>To examine the expression of hypoxia-inducible factor-1<i>α</i> (HIF-1<i>α</i>), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.<h4>Methods</h4>TfR1 and HIF-1<i>α</i> were detected by western blot. Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding.<h4>Results</h4>There was no difference in the expression of TfR1 and HIF-1<i>α</i> between groups. Lectin blot ana ...[more]

PMID: 30854239

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Project description:BACKGROUND:Previous studies have revealed that women with gestational diabetes mellitus (GDM) have an increased risk of developing preeclampsia (PE). The possible reason is the abnormal lipid metabolism caused by GDM that leads to dysfunction of vascular endothelial cells and atherosclerosis, resulting in the onset of PE. However, studies focusing on the pathogenesis of PE in syncytiotrophoblast of GDM patients are lacking. This study aimed to compare differentially expressed proteins from syncytiotrophoblast between women with GDM and women with GDM with subsequently developed PE. METHODS:Syncytiotrophoblast samples were obtained from pregnant women immediately after delivery. To explore the protein expression changes of syncytiotrophoblast that might explain the pathogenesis of PE in women with GDM, quantitative proteomics was performed using tandem mass tag (TMT) isobaric tags and liquid chromatography-tandem mass spectrometry. Bioinformatics analysis was performed to enrich the biological processes that these differentially expressed proteins were involved in. RESULTS:A total of 28,234 unique peptides and 4140 proteins were identified in all samples. Among them, 23 differentially expressed proteins were identified between patients with GDM and patients with GDM with subsequently developed PE. Therein, 11 proteins were upregulated and 12 proteins were downregulated. Two relative proteins (FLT1 and PABPC4) were independently verified using immunoblotting analysis. Bioinformatic results indicated that the onset of PE in patients with GDM is a multifactorial disorder, involving factors such as apoptosis, transcriptional misregulation, oxidative stress, lipid metabolism, cell infiltration and migration, and angiogenesis. CONCLUSION:These results indicated that the inadequacy of endometrium infiltration, angiogenic disorder, and oxidative stress in syncytiotrophoblast are more likely to occur in patients with GDM and may be the potential mechanisms leading to such patients secondarily developing severe early-onset PE.

Dataset Information

Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.

Objective

Methods

Results

Conclusion

Publications

Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.

Similar Datasets

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