Unknown

Dataset Information

0

A large CRISPR-induced bystander mutation causes immune dysregulation.


ABSTRACT: A persistent concern with CRISPR-Cas9 gene editing has been the potential to generate mutations at off-target genomic sites. While CRISPR-engineering mice to delete a ~360?bp intronic enhancer, here we discovered a founder line that had marked immune dysregulation caused by a 24?kb tandem duplication of the sequence adjacent to the on-target deletion. Our results suggest unintended repair of on-target genomic cuts can cause pathogenic "bystander" mutations that escape detection by routine targeted genotyping assays.

SUBMITTER: Simeonov DR 

PROVIDER: S-EPMC6379443 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

altmetric image

Publications


A persistent concern with CRISPR-Cas9 gene editing has been the potential to generate mutations at off-target genomic sites. While CRISPR-engineering mice to delete a ~360 bp intronic enhancer, here we discovered a founder line that had marked immune dysregulation caused by a 24 kb tandem duplication of the sequence adjacent to the on-target deletion. Our results suggest unintended repair of on-target genomic cuts can cause pathogenic "bystander" mutations that escape detection by routine target  ...[more]

Similar Datasets

| S-EPMC6839947 | biostudies-literature
| S-EPMC2234582 | biostudies-literature
2021-03-18 | PXD014163 | Pride
| S-EPMC1182269 | biostudies-literature
| S-EPMC5636941 | biostudies-literature
| S-EPMC7596814 | biostudies-literature
| S-EPMC10655813 | biostudies-literature
| S-EPMC6219745 | biostudies-literature
| S-EPMC10984984 | biostudies-literature
| S-EPMC7383251 | biostudies-literature