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Dietary supplementation with fermented Mao-tai lees beneficially affects gut microbiota structure and function in pigs.

ABSTRACT: Gut microbiota positively contribute to livestock nutrition and metabolism. The manipulation of these microbes may improve animal health. Some feed additives improve livestock health and metabolism by regulating gut microbiota composition and activity. We fed hybrid pigs diets supplemented with 0% (control), 5% (treat 1), 10% (treat 2), or 15% (treat 3) fermented Mao-tai lees (FML) for 90 days. Short-chain fatty acids (SCFAs), bioamines, and microbial communities found in colonic contents were analyzed to investigate microbiota composition and metabolic profiles. Concentrations of straight-chain fatty acids (e.g., acetate, propionate, and butyrate) and tyramine increased with FML supplementation content. Contrary to the minor effects of 5% and 10% FML on gut microbiota, 15% FML influenced beta diversity (Jaccard or Bray-Curtis dissimilarity) but not alpha diversity (number of operational taxonomic units and Shannon diversity) of pig gut microbial communities compared to the control group. Notably, 15% FML animals were characterized by a higher abundance of potentially beneficial bacteria (Lactobacillus and Akkermansia) but lower abundances of potential pathogens (Escherichia). Numerous genes associated with metabolism (e.g., starch, sucrose, and sulfur-compounds metabolism) showed a higher relative abundance in the 15% FML than in the control group. Additionally, most Phascolarctobacterium, Treponema, Prevotella, and Faecalibacterium bacterial markers in the 15% FML group were positively correlated with straight-chain fatty acid concentrations, suggesting that these bacteria are likely associated with SCFA production. Taken together, our findings demonstrate the beneficial effects of 15% FML on fermentation of undigested compounds and gut microbiota composition in the colon. Thus, 15% FML supplementation in pig feed may possibly represent a way to optimize pig colon health for livestock farming.

SUBMITTER: Li H

PROVIDER: S-EPMC6379501 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Dietary supplementation with fermented Mao-tai lees beneficially affects gut microbiota structure and function in pigs.

Li Huan H Li Huawei H Xie Peifeng P Li Zhihua Z Yin Yulong Y Blachier Francois F Kong Xiangfeng X

AMB Express 20190218 1

Gut microbiota positively contribute to livestock nutrition and metabolism. The manipulation of these microbes may improve animal health. Some feed additives improve livestock health and metabolism by regulating gut microbiota composition and activity. We fed hybrid pigs diets supplemented with 0% (control), 5% (treat 1), 10% (treat 2), or 15% (treat 3) fermented Mao-tai lees (FML) for 90 days. Short-chain fatty acids (SCFAs), bioamines, and microbial communities found in colonic contents were a ...[more]

PMID: 30778768

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Project description:This study investigated the effects of dietary supplementation with fermented Mao-tai lees (FML) on growth performance, plasma metabolites, and intestinal microbiota and metabolites of weaned piglets. A total of 128 Duroc×Landrace×Yorkshire piglets (28-days old) were randomly assigned to one of four groups, feeding a basal diet (control group), a basal diet supplemented with 2, 4 or 6% FML, respectively, for 42days. The results showed that dietary 4% FML supplementation had higher (p<0.05) average daily gain (ADG) and plasma triglyceride concentration during days 1-14 of the trial than the other FML supplemented groups. In addition, dietary 2 and 4% FML supplementation increased (p<0.05) the ADG during days 15-28 of the trial and plasma total protein concentration on day 42 of the trial compared with the 6% FML supplement. The plasma concentrations of arginine, ethanolamine, histidine, isoleucine, lysine, methionine, proline, taurine, threonine, and tyrosine were increased (p<0.05) in the 4% FML group compared with the other three groups on day 14 of the trial. Dietary supplementation with 2-6% FML decreased (p<0.05) the plasma urea nitrogen concentration on day 14 of the trial and the abundance of Escherichia coli in the colon, and dietary 2 and 4% FML supplementation decreased (p<0.05) the abundance of sulfate-reducing bacteria compared with the control group. In the intestinal contents, a higher concentration of FML (6%) supplementation decreased (p<0.05) the colonic acetate concentration compared with the control and 2% FML groups, while 4% FML supplementation increased (p<0.05) the colonic cadaverine concentration compared with the other three groups. In conclusion, dietary 4% FML supplementation might contribute to the increased amino acids metabolism without affecting the growth performance of weaned piglets. Moreover, dietary 2 and 4% FML supplementation were also beneficial to intestinal health via decreasing the abundances of specific pathogens and increasing the concentrations of microbial metabolites in the gut, which provides the theoretical basis and data support for the application of FML in pigs.

Project description:BackgroundAgaricus subrufescens is considered as one of the most important culinary-medicinal mushrooms around the world. It has been widely suggested to be used for the development of functional food ingredients to promote human health ascribed to the various properties (e.g., anti-inflammatory, antioxidant, and immunomodulatory activities). In this context, the interest in A. subrufescens based feed ingredients as alternatives for antibiotics has also been fuelled during an era of reduced/banned antibiotics use. This study aimed to investigate the effects of a fermented feed additive -rye overgrown with mycelium (ROM) of A. subrufescens-on pig intestinal microbiota, mucosal gene expression and local and systemic immunity during early life. Piglets received ROM or a tap water placebo (Ctrl) perorally every other day from day 2 after birth until 2 weeks post-weaning. Eight animals per treatment were euthanized and dissected on days 27, 44 and 70.ResultsThe results showed ROM piglets had a lower inter-individual variation of faecal microbiota composition before weaning and a lower relative abundance of proteobacterial genera in jejunum (Undibacterium and Solobacterium) and caecum (Intestinibacter and Succinivibrionaceae_UCG_001) on day 70, as compared to Ctrl piglets. ROM supplementation also influenced gut mucosal gene expression in both ileum and caecum on day 44. In ileum, ROM pigs showed increased expression of TJP1/ZO1 but decreased expression of CLDN3, CLDN5 and MUC2 than Ctrl pigs. Genes involved in TLR signalling (e.g., TICAM2, IRAK4 and LY96) were more expressed but MYD88 and TOLLIP were less expressed in ROM pigs than Ctrl animals. NOS2 and HIF1A involved in redox signalling were either decreased or increased in ROM pigs, respectively. In caecum, differentially expressed genes between two groups were mainly shown as increased expression (e.g., MUC2, PDGFRB, TOLLIP, TNFAIP3 and MYD88) in ROM pigs. Moreover, ROM animals showed higher NK cell activation in blood and enhanced IL-10 production in ex vivo stimulated MLN cells before weaning.ConclusionsCollectively, these results suggest that ROM supplementation in early life modulates gut microbiota and (local) immune system development. Consequently, ROM supplementation may contribute to improving health of pigs during the weaning transition period and reducing antibiotics use.

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Dietary supplementation with fermented Mao-tai lees beneficially affects gut microbiota structure and function in pigs.

Publications

Dietary supplementation with fermented Mao-tai lees beneficially affects gut microbiota structure and function in pigs.

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