Immunogenic Heterogeneity of Renal Cell Carcinoma With Venous Tumor Thrombus.

ABSTRACT:

Objective

To perform immune-cell enumeration and programmed death-ligand 1 (PD-L1) expression in clear cell renal cell carcinoma (cc-RCC) with tumor thrombus (TT) to guide therapeutic decisions.

Methods

After obtaining IRB approval and surgical consent, 6 patients underwent radical nephrectomy with venous tumor thrombectomy. We utilized RNA Sequencing to obtain RNAseq expression profiles. Computational calculation and enumeration of immune cells were performed using CIBERSORT, xCell, and ingenuity pathway analysis software. Statistical assessment was conducted using a t test, chi-square, ANOVA and Spearman rank correlations using SPSS v21.

Results

We observed a higher proportion of M1 macrophages in the primary tumor and tumor thrombus, while we noted no difference in

SUBMITTER: Liss MA 

PROVIDER: S-EPMC6382529 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature