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Identification of a Xist silencing domain by Tiling CRISPR.

ABSTRACT: Despite essential roles played by long noncoding RNAs (lncRNAs) in development and disease, methods to determine lncRNA cis-elements are lacking. Here, we developed a screening method named "Tiling CRISPR" to identify lncRNA functional domains. Using this approach, we identified Xist A-Repeats as the silencing domain, an observation in agreement with published work, suggesting Tiling CRISPR feasibility. Mechanistic analysis suggested a novel function for Xist A-repeats in promoting Xist transcription. Overall, our method allows mapping of lncRNA functional domains in an unbiased and potentially high-throughput manner to facilitate the understanding of lncRNA functions.

SUBMITTER: Wang Y

PROVIDER: S-EPMC6382781 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Identification of a Xist silencing domain by Tiling CRISPR.

Wang Yang Y Zhong Yang Y Zhou Yingyao Y Tanaseichuk Olga O Li Zhizhong Z Zhao Jing Crystal JC

Scientific reports 20190220 1

Despite essential roles played by long noncoding RNAs (lncRNAs) in development and disease, methods to determine lncRNA cis-elements are lacking. Here, we developed a screening method named "Tiling CRISPR" to identify lncRNA functional domains. Using this approach, we identified Xist A-Repeats as the silencing domain, an observation in agreement with published work, suggesting Tiling CRISPR feasibility. Mechanistic analysis suggested a novel function for Xist A-repeats in promoting Xist transcri ...[more]

PMID: 30787302

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