Dataset Information

Characterization of Dye Extracts from Historical Cultural-Heritage Objects Using State-of-the-Art Comprehensive Two-Dimensional Liquid Chromatography and Mass Spectrometry with Active Modulation and Optimized Shifting Gradients.

ABSTRACT: Unbiased characterization of dyes and their degradation products in cultural-heritage objects requires an analytical method which provides universal separation power regardless of dye classes. Dyes are small molecules that vary widely in chemical structure and properties, which renders their characterization by a single method challenging. We have developed a comprehensive two-dimensional liquid chromatography method hyphenated with mass spectrometry and UV-vis detection. We use stationary-phase-assisted modulation to enhance the method in terms of detection limits and solvent compatibility and to reduce the analysis time. The PIOTR program was used to optimize an assembly of shifting second-dimension gradients, which resulted in a high degree of orthogonality (80% in terms of the asterisk concept). The resulting method is universally applicable to all classes of dyes extracted from cultural-heritage objects. Thanks to the high peak capacity and orthogonality, dye components can be separated from chemically similar impurities and degradation products, providing a detailed fingerprint of the dyes mixture in a specific sample. The method was applied to a number of challenging dye extracts from 17th- and 19th-century cultural-heritage objects.

SUBMITTER: Pirok BWJ

PROVIDER: S-EPMC6383186 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Publications

Characterization of Dye Extracts from Historical Cultural-Heritage Objects Using State-of-the-Art Comprehensive Two-Dimensional Liquid Chromatography and Mass Spectrometry with Active Modulation and Optimized Shifting Gradients.

Pirok Bob W J BWJ den Uijl Mimi J MJ Moro Giacomo G Berbers Sanne V J SVJ Croes Charlotte J M CJM van Bommel Maarten R MR Schoenmakers Peter J PJ

Analytical chemistry 20190201 4

Unbiased characterization of dyes and their degradation products in cultural-heritage objects requires an analytical method which provides universal separation power regardless of dye classes. Dyes are small molecules that vary widely in chemical structure and properties, which renders their characterization by a single method challenging. We have developed a comprehensive two-dimensional liquid chromatography method hyphenated with mass spectrometry and UV-vis detection. We use stationary-phase ...[more]

PMID: 30650969

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Project description:In this initial attempt to identify fungi predominantly colonizing art objects, mural paintings and a bas-relief, at 12 archaeological sites in the central and western parts of Thailand, 13 fungal isolates were identified using morphological technique and estimated for their prevalence frequency at each site. Five main genera of fungal community found were Aspergillus, Fusarium, Curvularia, Penicillium, and Neurospora. These fungi were further identified to species level by molecular method utilizing nucleotide sequence homology analysis of the conserved internal transcribed spacer (ITS) region. Environmental factors such as temperature, relative humidity, and the opening or closure of the temples did not have any influence upon the fungal type. From the area-based distribution, Aspergillus was found at all collection sites, while Fusarium was found in Bangkok, and Ratchaburi and Petchaburi provinces in the western part of the country. Curvularia was found mostly in Phra Nakhon Si Ayutthaya and Lopburi provinces, and in one temple in Petchaburi. From the phylogenetic relationship, these prevalent fungi were divided into three closely related groups: Aspergillus and Penicillium, Fusarium and Neurospora, and Curvularia. In addition, growth inhibition of the fungi by local plant extracts of betel leaves, custard apple leaves, mangosteen peel, and guava leaves at 10,000 ppm were investigated. Mangosteen peel extract gave the highest fungal growth inhibition for all the Curvularia tested, being 68.3%, 65.6%, and 60% for C. verruculosa, C. geniculata, and C. lunata, respectively. Guava leave extract also yielded highest growth inhibition of 64.4% for C. verruculosa. Both betel leave and custard apple leave extracts showed the highest inhibition towards A. fumigatus, at 65.1% and 61.8%, respectively. The results obtained here are basic information necessary for future applications in the biological prevention of art objects, and the design of appropriate measures for preventive conservation of Thai cultural heritage.

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Dataset Information

Characterization of Dye Extracts from Historical Cultural-Heritage Objects Using State-of-the-Art Comprehensive Two-Dimensional Liquid Chromatography and Mass Spectrometry with Active Modulation and Optimized Shifting Gradients.

Publications

Characterization of Dye Extracts from Historical Cultural-Heritage Objects Using State-of-the-Art Comprehensive Two-Dimensional Liquid Chromatography and Mass Spectrometry with Active Modulation and Optimized Shifting Gradients.

Similar Datasets

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