A new approach for Small Ruminant Lentivirus full genome characterization revealed the circulation of divergent strains.

ABSTRACT: Small Ruminant Lentiviruses (SRLV) include at least 4 viral highly divergent genotypes. Genotypes A and B are widely distributed and genotypes C and E have been recognized in restricted geographic areas. New phylogroups have been identified targeting conserved regions. However, this approach suffers from the potential risk to misamplify highly divergent strains. Pathogenic strains are easily adapted to fibroblastic cells, but non-pathogenic strains isolation may require a different approach. We developed a fast and effective method for SRLV full genome characterization after cell culture isolation. Spleen samples were collected during regular slaughter from sheep and goats in northwestern Italy. Spleen-derived macrophage cultures were monitored for reverse transcriptase activity and RNA wa

SUBMITTER: Colitti B 

PROVIDER: S-EPMC6383919 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature