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Photoacoustic transfection of DNA encoding GFP.

ABSTRACT: Photoacoustic transfection consists in the use of photoacoustic waves, generated in the thermoelastic expansion of a confined material absorbing a short pulse of a laser, to produce temporary mechanical deformations of the cell membrane and facilitate the delivery of plasmid DNA into cells. We show that high stress gradients, produced when picosecond laser pulses with a fluence of 100 mJ/cm² are absorbed by piezophotonic materials, enable transfection of a plasmid DNA encoding Green Fluorescent Protein (gWizGFP, 3.74 MDa) in COS-7 monkey fibroblast cells with an efficiency of 5% at 20 °C, in 10 minutes. We did not observe significant cytotoxicity under these conditions. Photoacoustic transfection is scalable, affordable, enables nuclear localization and the dosage is easily controlled by the laser parameters.

SUBMITTER: Silva AD

PROVIDER: S-EPMC6384874 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Photoacoustic transfection of DNA encoding GFP.

Silva Alexandre D AD Serpa Carlos C Arnaut Luis G LG

Scientific reports 20190222 1

Photoacoustic transfection consists in the use of photoacoustic waves, generated in the thermoelastic expansion of a confined material absorbing a short pulse of a laser, to produce temporary mechanical deformations of the cell membrane and facilitate the delivery of plasmid DNA into cells. We show that high stress gradients, produced when picosecond laser pulses with a fluence of 100 mJ/cm<sup>2</sup> are absorbed by piezophotonic materials, enable transfection of a plasmid DNA encoding Green F ...[more]

PMID: 30796229

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