Ontology highlight
ABSTRACT:
SUBMITTER: Tomatis PE
PROVIDER: S-EPMC6384906 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Tomatis Pablo Emiliano PE Schütz Marco M Umudumov Elina E Plückthun Andreas A
Scientific reports 20190221 1
Eukaryotic integral membrane proteins (IMPs) are difficult to study due to low functional expression levels. To investigate factors for efficient biogenesis of eukaryotic IMPs in the prokaryotic model organism Escherichia coli, important, e.g., for isotope-labeling for NMR, we selected for E. coli cells expressing high levels of functional G protein-coupled receptors (GPCRs) by FACS. Utilizing an E. coli strain library with all non-essential genes systematically deleted, we unexpectedly discover ...[more]