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Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM.

ABSTRACT: Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring. CSF showed ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan. The amounts and disaccharide composition of these GAGs differed from those found in human plasma. This approach may offer a new method for the discovery of biomarkers for diseases of the central nervous system.

SUBMITTER: Yu Y

PROVIDER: S-EPMC6386465 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM.

Yu Yanlei Y Zhang Fuming F Colón Wilfredo W Linhardt Robert J RJ Xia Ke K

Analytical biochemistry 20181218

Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring. CSF showed ng/mL levels of heparan sulfate, chondroitin sulfates and hyaluronan. The amounts and disaccharide composition of these GAGs differed from those found in human plasma. This approach may offer a new method for the discovery of biomarkers ...[more]

PMID: 30571946

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