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Exogenous Application of Phytohormones Promotes Growth and Regulates Expression of Wood Formation-Related Genes in Populus simonii × P. nigra.


ABSTRACT: Although phytohormones are known to be important signal molecules involved in wood formation, their roles are still largely unclear. Here, Populus simonii × P. nigra seedlings were treated with different concentrations of exogenous phytohormones, indole-3-acetic acid (IAA), gibberellin (GA₃), and brassinosteroid (BR), and the effects of phytohormones on growth were investigated. Next, 27 genes with known roles in wood formation were selected for qPCR analysis to determine tissue-specificity and timing of responses to phytohormone treatments. Compared to the control, most IAA, GA₃, and BR concentrations significantly increased seedling height. Meanwhile, IAA induced significant seedling stem diameter and cellulose content increases that peaked at 3 and 30 mg·L-1, respectively. Significant increase in cellulose content was also observed in seedlings treated with 100 mg·L-1 GA₃. Neither stem diameter nor cellulose content of seedlings were affected by BR treatment significantly, although slight effects were observed. Anatomical measurements demonstrated improved xylem, but not phloem, development in IAA- and BR-treated seedlings. Most gene expression patterns induced by IAA, GA₃, and BR differed among tissues. Many IAA response genes were also regulated by GA₃, while BR-induced transcription was weaker and slower in Populus than for IAA and GA₃. These results reveal the roles played by phytohormones in plant growth and lay the foundation for exploring molecular regulatory mechanisms of wood formation in Populus.

SUBMITTER: Yuan H 

PROVIDER: S-EPMC6387376 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Exogenous Application of Phytohormones Promotes Growth and Regulates Expression of Wood Formation-Related Genes in <i>Populus simonii</i> × <i>P. nigra</i>.

Yuan Hongmei H   Zhao Lijuan L   Guo Wendong W   Yu Ying Y   Tao Lei L   Zhang Liguo L   Song Xixia X   Huang Wengong W   Cheng Lili L   Chen Jing J   Guan Fengzhi F   Wu Guangwen G   Li Huiyu H  

International journal of molecular sciences 20190212 3


Although phytohormones are known to be important signal molecules involved in wood formation, their roles are still largely unclear. Here, <i>Populus simonii</i> × <i>P. nigra</i> seedlings were treated with different concentrations of exogenous phytohormones, indole-3-acetic acid (IAA), gibberellin (GA₃), and brassinosteroid (BR), and the effects of phytohormones on growth were investigated. Next, 27 genes with known roles in wood formation were selected for qPCR analysis to determine tissue-sp  ...[more]

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